Anti-Integrin β5/ITGB5 Monoclonal Antibody for FC - P18084

Core Product Specifications and Parameters

Biological Background: Integrin beta-5 Function and Localization

• Integrin beta-5 (ITGB5) is the beta subunit of the integrin alpha-V/beta-5 heterodimer, a cell surface receptor for fibronectin. It specifically recognizes the RGD (Arg-Gly-Asp) sequence in its ligands, mediating cell adhesion and migration.
• In the context of host-pathogen interactions, the integrin alpha-V/beta-5 complex serves as a receptor for adenovirus type C entry into host cells.
• The protein is localized at the cell membrane, consistent with its role in signal transduction from the extracellular matrix to the intracellular environment.
• Key structural features include an extracellular domain with EGF-like repeats, a single-pass transmembrane helix, and a short cytoplasmic tail, indicative of typical integrin beta subunit architecture.
• Post-translational modifications include phosphorylation, which may regulate integrin activation and downstream signaling, and disulfide bond formation, contributing to domain stability.
• No tissue-specific expression data is available from the provided uniprot record, though integrins are broadly expressed in various cell types.
• The protein is classified under several keywords: Calcium, Cell adhesion, Cell membrane, Disulfide bond, EGF-like domain, Glycoprotein, Host cell receptor for virus entry, Host-virus interaction, Integrin, Metal-binding, Membrane, Phosphoprotein, Receptor, Reference proteome, Repeat, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the extracellular region (amino acids 24-490) of human ITGB5, which includes the fibronectin-binding domain. This design likely targets the native, extracellular conformation of the protein, making it suitable for flow cytometry (FC) applications that detect surface antigens.
• For FC, use live, unfixed cells to preserve surface epitope integrity. Perform antibody titrations to determine optimal staining concentrations for your specific cell line. Include appropriate isotype controls and ensure Fc receptor blocking if necessary.
• Since this antibody is validated for FC only, for other applications such as Western blot or immunohistochemistry, experimental validation under the relevant conditions is recommended.
• Integrin heterodimers can be sensitive to divalent cation concentrations; maintaining calcium and magnesium in staining buffers may be important for preserving the alpha-V/beta-5 complex and antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including monoclonal antibodies like this anti-ITGB5 rabbit mAb, designed for adhesion and viral entry research. In addition to individual antibody reagents, CamelBio can supply matched pairs, bulk ancillary reagents, and assist with sourcing rare targets—streamlining your workflow from early-stage assay development to clinical application. Reach out to explore how we can tailor sourcing solutions for your ITGB5-related projects.