Anti-Integrin-β1/CD29 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05556

Core Product Specifications and Parameters

Biological Background: Integrin-β1 Function and Localization

• Integrin beta-1 (CD29) is a multi-pass transmembrane glycoprotein that pairs with various alpha subunits to form functional heterodimeric receptors for extracellular matrix ligands.
• Beta-1 integrins mediate cell adhesion by recognizing RGD sequences in fibronectin, fibrinogen, vitronectin, laminin, and other ECM proteins.
• The α4/β1 integrin binds fractalkine (CX3CL1) and may serve as a co-receptor in CX3CR1-dependent signaling. Related references: PMID:23125415, PMID:24789099
• The α5/β1 integrin acts as a receptor for fibronectin and fibrillin-1, mediating RGD-dependent cell adhesion critical for matrix assembly. Related references: PMID:33962943, PMID:12807887, PMID:17158881
• α5/β1 integrin functions as a receptor for interleukin-1 beta (IL1B), and this interaction is essential for IL1B signal transduction. Related references: PMID:29030430
• The protein localizes to the plasma membrane, cell projections (invadopodia, lamellipodia, ruffles), focal adhesions, and the cleavage furrow, consistent with roles in adhesion, migration, and cytokinesis.
• Integrin beta-1 is widely expressed in tissues including placenta, skin, liver, skeletal muscle, and cardiac muscle, with specific isoforms showing restricted distributions. Related references: PMID:33962943, PMID:1551917, PMID:7545396, PMID:7681433
• High-throughput proteomics has identified acetylation, phosphorylation, and ubiquitination sites, suggesting regulation by multiple signaling pathways.

Experimental Guidance and Technical Tips

• In Western blotting, expect a band near 88 kDa; include positive controls from known integrin-expressing cell lines and optimize antibody dilution for your specific samples.
• For immunofluorescence/immunocytochemistry, optimize permeabilization conditions as CD29 is a transmembrane protein; co-staining with membrane or focal adhesion markers may aid localization studies.
• The antibody is suitable for ELISA; consider coating with the recombinant immunogen or native protein as a positive control and validate linearity and sensitivity in your assay setup.
• Given its cross-reactivity with mouse and rat, this antibody can be employed in animal model studies; however, confirm specificity with appropriate knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This rabbit polyclonal antibody against Integrin-β1/CD29 supports investigations into cell adhesion, migration, and integrin signaling pathways. In addition to validated antibodies, CamelBio offers optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.