Anti-Integrin-β1/CD29 Monoclonal Antibody for IF/ICC, ELISA - P05556

Core Product Specifications and Parameters

Biological Background: Integrin-β1 Function and Localization

• Integrin beta-1 (ITGB1), also known as fibronectin receptor subunit beta or CD29, is a transmembrane receptor that pairs with various alpha subunits to form functional integrin heterodimers. It serves as a receptor for collagen, fibronectin, laminin, vitronectin, and other extracellular matrix proteins, recognizing R-G-D motifs in many ligands.
• ITGA4:ITGB1 acts as a coreceptor for fractalkine (CX3CL1), while ITGA5:ITGB1 and ITGA4:ITGB1 bind PLA2G2A, modulating integrin conformational changes and enhancing ligand binding. Related references: PMID:23125415 PMID:24789099 PMID:18635536 PMID:25398877
• It plays pivotal roles in cell adhesion, migration, and signaling, contributing to processes such as angiogenesis, osteoblast compaction, cytokinesis, and myoblast differentiation.
• Integrin beta-1 is widely expressed, with high levels in placenta, vascular smooth muscle, and striated muscle. Alternative splicing generates multiple isoforms; for example, isoform 5 is specifically expressed in striated muscle and can displace isoform 1.
• Subcellularly, it localizes to the cell membrane, focal adhesions, lamellipodia, invadopodia, cleavage furrow, and sarcolemma, reflecting its dynamic involvement in adhesion turnover and movement.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which may regulate its activity, stability, and interactions.
• Beyond host physiology, Integrin beta-1 serves as a receptor for many viruses (e.g., echovirus, rotavirus, Epstein-Barr virus) and mediates fungal invasion during mucormycosis via interaction with CotH proteins. Related references: PMID:32487760

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular domain (aa 1-728), allowing recognition of native conformations. For IF/ICC, surface staining can be performed on live or lightly fixed cells; permeabilization may be needed for intracellular pools. Validate staining under relevant conditions.
• For ELISA, consider pairing this monoclonal with a detection antibody against a non-overlapping epitope to avoid competition, particularly in sandwich formats. Pilot experiments are recommended to determine optimal dilutions.
• The antibody cross-reacts with mouse and rat Integrin-β1, making it suitable for rodent model studies. Confirm specificity in the target tissue and species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Integrin-β1 antibody supports integrin-related research and assay development, covering applications from cell adhesion to host-pathogen interaction studies. For rare target sourcing or custom development needs, trust CamelBio to bridge your concept-to-clinic requirements efficiently.