Anti-Integrin beta 8 Monoclonal Antibody for WB, ELISA - P26012

Core Product Specifications and Parameters

Biological Background: Integrin beta-8 Function and Localization

• Integrin beta-8 (ITGB8) is a member of the integrin beta chain family, serving as a subunit for heterodimeric receptors that mediate cell-extracellular matrix interactions and signal transduction.
• As part of the integrin alpha-V:beta-8 (ITGAV:ITGB8) complex, it functions as a receptor for fibronectin, recognizing the R-G-D sequence within its ligands. Related references: PMID:1918072
• The alpha-V:beta-8 integrin is involved in the activation of transforming growth factor beta-1 (TGF-beta-1) by mediating R-G-D-dependent release from the latency-associated peptide (LAP), critical for immune regulation on regulatory T-cells (Tregs).
• Required during vasculogenesis, indicating a role in the development of blood vessels.
• Localized predominantly to the cell membrane, consistent with its function as a cell surface receptor.
• Exhibits tissue-specific expression in placenta, kidney, brain, ovary, uterus, and various transformed cell lines; transiently expressed in 293 human embryonic kidney cells.
• Contains characteristic integrin structural features, including EGF-like domains, disulfide bonds, and metal-binding sites for calcium and magnesium, essential for receptor conformation and ligand binding.
• Alternative splicing generates multiple isoforms, potentially diversifying its functional repertoire.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 43-320 of the extracellular region, covering the N-terminal ligand-binding domains; consider this when designing blocking peptides or verifying epitope specificity.
• For Western blot, both predicted isoforms (71 kDa and 86 kDa) may be detected; optimize protein loading and detection conditions to resolve these bands clearly.
• In ELISA applications, ensure proper coating of recombinant ITGB8 protein or cell-derived extracts; validate assay conditions with positive and negative controls.
• The antibody’s cross-reactivity with human, mouse, and rat enables multispecies studies; verify reactivity in the relevant sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a one-stop pipeline for IVD raw materials, technical services, and consulting. For integrin beta-8 and cell adhesion research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing—helping you streamline assay development from concept to clinical application.