Anti-Integrin beta 4 (ITGB4/CD104) Rabbit Polyclonal Antibody for WB, ELISA - P16144

Core Product Specifications and Parameters

Biological Background: Integrin beta-4 Function and Localization

• Integrin beta-4 (ITGB4), also known as CD104 or GP150, is a subunit of the integrin alpha-6/beta-4 heterodimer, which functions as a receptor for laminin.
• The alpha-6/beta-4 integrin plays a critical structural role in hemidesmosomes of epithelial cells, essential for keratinocyte polarity and motility.
• ITGA6:ITGB4 binds to NRG1 (via EGF domain), and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). Related references: PMID:20682778
• ITGA6:ITGB4 binds to IGF1, and this interaction is essential for IGF1 signaling (PubMed:22351760). Related references: PMID:22351760
• ITGA6:ITGB4 also binds to IGF2, which is essential for IGF2 signaling (PubMed:28873464). Related references: PMID:28873464
• Isoform beta-4D is expressed in colon and placenta, while isoform beta-4E is found in epidermis, lung, duodenum, heart, spleen, and stomach, indicating tissue-specific roles.
• The protein localizes to the cell membrane and is enriched at hemidesmosome cell junctions.
• Structural features include EGF-like domains, disulfide bonds, and potential palmitoylation and phosphorylation sites, which may regulate its function and localization.
• Mutations in ITGB4 are associated with epidermolysis bullosa, highlighting its importance in skin integrity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 611-710 in the extracellular domain of human ITGB4; this region may be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band around 202 kDa is expected; inclusion of a reducing agent and adequate transfer time for high molecular weight proteins is recommended.
• As a positive control, epithelial cell lines (e.g., HaCaT, A431) with high ITGB4 expression can be employed.
• For ELISA, the recombinant protein used as immunogen can serve as a coating antigen; titrate the antibody for optimal performance.
• Polyclonal antibodies may exhibit lot-to-lot variability; validate performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, offering a comprehensive range of validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-ITGB4 polyclonal antibody supports research in cell adhesion, epithelial biology, and hemidesmosome-related applications. With expertise in rare-target sourcing, we help bridge the gap from concept to clinic.