Anti-Integrin beta 4 (ITGB4/CD104) Monoclonal Antibody for WB, ELISA - P16144

Core Product Specifications and Parameters

Biological Background: Integrin beta-4 Function and Localization

• Integrin alpha-6/beta-4 serves as a receptor for laminin, playing a critical structural role in the hemidesmosome of epithelial cells and regulating keratinocyte polarity and motility.
• ITGA6:ITGB4 binds to NRG1 (via EGF domain), and this interaction is essential for NRG1-ERBB signaling. Related references: PMID:20682778
• ITGA6:ITGB4 binds to IGF1, an interaction essential for IGF1 signaling. Related references: PMID:22351760
• ITGA6:ITGB4 binds to IGF2, an interaction essential for IGF2 signaling. Related references: PMID:28873464
• Subcellularly localized to the cell membrane, cell junction, and hemidesmosome.
• Predominantly expressed in epithelia; isoform beta-4D is additionally found in colon and placenta, while isoform beta-4E is expressed in epidermis, lung, duodenum, heart, spleen, and stomach.
• Keywords from UniProt: alternative splicing, calcium binding, cell adhesion, EGF-like domain, glycoprotein, disulfide bond, phosphoprotein, receptor, and disease variants in epidermolysis bullosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1604–1822), which may allow detection of multiple ITGB4 isoforms; consider validating band patterns in your experimental system.
• For Western blot, the predicted molecular weight is ~202 kDa; use appropriate high-molecular-weight protein markers and optimized gel conditions.
• For ELISA, the recombinant immunogen can serve as a positive control for assay development.
• Due to reported cross-reactivity with mouse, validate the antibody’s performance in mouse epithelial cell lines or tissue lysates.
• Given the protein’s extensive disulfide bonding and phosphorylation, reducing and non-reducing SDS-PAGE conditions may resolve different forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers streamlined access to validated IVD raw materials, including monoclonal antibodies like this anti-ITGB4 clone for epithelial biology and cancer research. Our portfolio spans optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—designed to support your assay development, manufacturing scale-up, and procurement efficiency.