Anti-Integrin beta 4 (ITGB4/CD104) Monoclonal Antibody for IF-P, IHC-P, ELISA - P16144

Core Product Specifications and Parameters

Biological Background: Integrin beta-4 Function and Localization

• Integrin beta-4 (ITGB4), also known as CD104 or GP150, is a single-pass type I membrane protein with a molecular weight of approximately 202 kDa.
• It functions as a receptor for laminin by forming a heterodimer with integrin alpha-6, and plays a critical structural role in the hemidesmosome of epithelial cells, regulating keratinocyte polarity and motility.
• ITGA6:ITGB4 interacts with NRG1 via its EGF domain, an interaction essential for NRG1-ERBB signaling. Related references: PMID:20682778
• ITGA6:ITGB4 binds to IGF1, a step required for IGF1 signaling. Related references: PMID:22351760
• ITGA6:ITGB4 binds to IGF2, an interaction critical for IGF2 signaling. Related references: PMID:28873464
• The protein localizes to the cell membrane and is enriched at cell junctions, specifically within hemidesmosomes.
• It is predominantly expressed in epithelia, with additional isoforms detected in colon, placenta, epidermis, lung, duodenum, heart, spleen, and stomach.
• The integrin beta-4 sequence contains disulfide bonds, EGF-like domains, and undergoes post-translational modifications including glycosylation, palmitoylation, and phosphorylation; it is involved in cell adhesion and integrin-mediated signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 611-710 of human Integrin β4 (NP_000204.3), which lies within the extracellular domain. This region may be suitable for detecting both full-length and certain truncated or shed forms; however, validation in your specific sample type is recommended.
• For IF-P and IHC-P, antigen retrieval conditions (e.g., citrate buffer, pH 6.0) and permeabilization steps should be optimized to ensure accessible epitopes in paraffin-embedded sections.
• For ELISA, consider using an indirect format with the antibody as the detection reagent after coating with recombinant Integrin β4 or cell lysates. Pairing with a capture antibody may enhance sensitivity.
• Cross-reactivity is reported with mouse and rat, but due to potential species-specific sequence variations, confirm reactivity if precise quantification or comparative studies are planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to validated IVD raw materials, including monoclonal antibodies like this anti-Integrin beta-4 Rabbit mAb, ideal for cell adhesion and epithelial biology studies. As a one-stop partner from concept to clinic, we offer ready-to-use antibody pairs, bulk ancillary reagents, and sourcing of rare targets to streamline your assay development and production needs. Explore our portfolio to accelerate your next integrin-focused diagnostic or research project.