Anti-Integrin beta 3 (ITGB3/CD61) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05106

Core Product Specifications and Parameters

Biological Background: Integrin beta-3 Function and Localization

• Integrin beta-3 (ITGB3), also known as GPIIIa or CD61, is a single-pass type I membrane protein that serves as the beta subunit of integrin heterodimers αVβ3 and αIIbβ3.
• The αIIbβ3 complex is crucial for platelet aggregation; upon activation, it binds soluble fibrinogen to mediate platelet-platelet interactions, a step essential for hemostatic plug formation (PubMed:9111081). Related references: PMID:9111081
• β3 integrins recognize the R-G-D sequence present in multiple extracellular matrix ligands, including fibronectin, fibrinogen, vitronectin, and thrombospondin, thereby regulating cell adhesion, migration, and outside-in signaling.
• Integrin αVβ3 also functions as a coreceptor for growth factor signaling (e.g., FGF1/FGF2, IGF1/IGF2, NRG1, IL1B) and participates in synaptic plasticity by controlling AMPAR endocytosis and serotonin reuptake in the brain. Related references: PMID:18441324, PMID:28302677, PMID:19578119, PMID:28873464, PMID:20682778, PMID:29030430
• Additionally, ITGB3 serves as a receptor for various viruses, including herpesvirus 8, coxsackievirus A9, hantavirus, cytomegalovirus, and human metapneumovirus, facilitating host cell entry.
• The protein localizes to the plasma membrane, enriched at lamellipodia, focal adhesions, and postsynaptic membranes, consistent with its roles in adhesion and signaling.
• Isoform beta-3A is widely expressed, while isoform beta-3C is specifically detected in prostate and testis; this tissue specificity may underlie distinct biological functions.
• Key structural features include an EGF-like domain, a signal peptide, a large extracellular region with metal-binding sites and disulfide bonds, and a short cytoplasmic tail subject to phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80-430 of human ITGB3, covering a large portion of the extracellular domain; this design may enable recognition of both native and denatured protein.
• For western blotting, a band near 87 kDa is expected under reducing conditions; consider using a 5% non-fat milk blocking buffer to minimize non-specific binding.
• In immunohistochemistry (IHC-P), perform antigen retrieval using citrate buffer (pH 6.0) and validate staining in known positive tissues such as platelets or osteoblasts.
• For ELISA, the antibody might be applicable as a capture or detection reagent; optimization of coating conditions and detection range is essential.
• Given the reported cross-reactivity with mouse and rat, this antibody can be utilized in rodent models of thrombosis, cell adhesion, and integrin signaling.
• Avoid repeated freeze-thaw cycles and store at -20°C upon arrival to maintain stability; the product is shipped on ice bags.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibodies for integrin targets like ITGB3. We offer optimized polyclonal and monoclonal antibodies, bulk reagent supply, and custom sourcing solutions for your platelet biology and cell adhesion research needs. From single antibodies to integrated assay development support, CamelBio is your dedicated partner from concept to clinic.