Anti-Integrin beta 3 (ITGB3/CD61) Rabbit Monoclonal Antibody for WB, IP, FC, ELISA - P05106

Core Product Specifications and Parameters

Biological Background: Integrin beta-3 Function and Localization

• Integrin beta-3 (ITGB3), also known as platelet glycoprotein IIIa (GPIIIa) or CD61, is a transmembrane receptor subunit that pairs with integrin alpha-V (ITGAV) or alpha-IIb (ITGA2B) to form heterodimeric integrins. Two major isoforms exist: beta-3A is widely expressed and specifically in osteoblasts, while beta-3C is restricted to prostate and testis.
• As part of integrin alpha-IIb/beta-3, it mediates platelet aggregation by binding soluble fibrinogen, a step critical for rapid platelet plug formation. Related references: PMID:9111081
• Both alpha-V/beta-3 and alpha-IIb/beta-3 recognize the R-G-D sequence in multiple ligands including fibronectin, vitronectin, osteopontin, and thrombospondin, enabling cell adhesion and migration.
• ITGB3 localizes to the cell membrane, lamellipodia, focal adhesions, and postsynaptic compartments, supporting roles in cell adhesion, spreading, and synaptic plasticity.
• In the brain, ITGAV:ITGB3 regulates serotonin neurotransmission by controlling SLC6A4 localization and modulates AMPAR endocytosis, thereby influencing synaptic strength.
• ITGAV:ITGB3 acts as a co-receptor or direct binding partner for multiple growth factors, including FGF1, FGF2, IGF1, IGF2, and IL1B, facilitating their signaling pathways. Related references: PMID:18441324 PMID:28302677 PMID:19578119 PMID:28873464 PMID:29030430
• ITGB3 serves as a host cell receptor for various viruses, such as Herpesvirus 8, Coxsackievirus A9, Hantaan virus, and West Nile virus, highlighting its role in microbial pathogenesis.
• The protein undergoes phosphorylation and contains disulfide bonds and EGF-like domains; these modifications may regulate integrin activation and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal intracellular peptide (aa 689-788); verify that the sequence is conserved across isoforms of interest to ensure broad detection.
• For western blot, the predicted molecular weight is approximately 87 kDa; glycosylation may cause a higher apparent mass. Validate using platelet lysates or ITGB3-expressing cell lines.
• In immunoprecipitation, capture of native ITGB3 complexes may be possible; confirm co-precipitation of alpha integrin partners in the relevant sample system.
• For flow cytometry (intracellular), permeabilization is required; titrate the antibody and include appropriate isotypic controls to minimize background.
• ELISA applications may be suitable for detecting recombinant or native ITGB3; pairing with another clone or using as a capture antibody could be explored for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, including this rabbit monoclonal anti-ITGB3 antibody suitable for platelet biology and integrin signaling research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—streamlining your assay development from concept to clinic. Our expertise spans rare target procurement, supporting your diagnostic and research needs with reliable, scalable solutions.