Anti-Integrin alpha V (ITGAV/CD51) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P06756

Core Product Specifications and Parameters

Biological Background: Integrin alpha-V Function and Localization

• Integrin alpha-V (ITGAV) pairs with various beta subunits (e.g., ITGB3, ITGB5, ITGB6, ITGB8) to form heterodimeric receptors that recognize the RGD motif in vitronectin, fibronectin, fibrinogen, osteopontin, and numerous other ligands.
• ITGAV:ITGB3 binds to fractalkine (CX3CL1), NRG1, FGF1, FGF2, IGF1, IGF2, IL1B, and CD40LG, modulating chemokine, growth factor, and cytokine signaling pathways. Related references: PMID:23125415, PMID:20682778, PMID:18441324, PMID:28302677, PMID:19578119, PMID:28873464, PMID:29030430, PMID:31331973
• ITGAV:ITGB6 and ITGAV:ITGB8 mediate RGD-dependent release of TGF-beta-1 from latency-associated peptide (LAP), playing a key role in TGF-beta activation. Related references: PMID:15184403, PMID:22278742, PMID:28117447
• ITGAV:ITGB3 binds to PLA2G2A via a distinct site (site 2), inducing integrin conformational changes and enhanced ligand binding to the classical site. Related references: PMID:18635536, PMID:25398877
• ITGAV:ITGB3 and ITGAV:ITGB6 function as receptors for fibrillin-1 (FBN1) and mediate RGD-dependent cell adhesion to FBN1. Related references: PMID:12807887, PMID:17158881
• ITGAV:ITGB3 acts as a receptor for bone sialoprotein (IBSP) and promotes cell adhesion and migration. Related references: PMID:10640428
• Integrin alpha-V localizes to the cell membrane and focal adhesions; its large extracellular domain contains disulfide bonds, glycosylation sites, and calcium-binding repeats.
• ITGAV-containing integrins serve as host cell receptors for numerous viruses, including Adenovirus, Coxsackievirus, herpes simplex virus, and HIV-1, contributing to viral entry and pathogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal cytoplasmic region (aa 950‑1048) of human ITGAV; the antibody is expected to detect the full‑length protein (~116 kDa) and may also recognize alternative isoforms or cleavage products.
• For Western blot (WB), validate the antibody in lysates from ITGAV‑expressing cell lines (e.g., M21 melanoma, HUVEC) and confirm the band size; optimize dilution and blocking conditions.
• In immunohistochemistry (IHC‑P), antigen retrieval may be required; include appropriate tissue controls and verify staining specificity using a blocking peptide or knockout model.
• For immunoprecipitation (IP) and ELISA, consider testing different antibody concentrations and pairing with suitable detection reagents to ensure reproducible results.
• As a rabbit polyclonal antibody, lot‑to‑lot variability should be assessed; validate critical applications with each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw material procurement, offering validated antibodies, matching antigen pairs, and bulk ancillary reagents to accelerate diagnostic development. This anti‑ITGAV (CD51) rabbit polyclonal antibody supports cell adhesion, integrin signaling, and viral infection research—backed by CamelBio’s commitment to quality supply and technical support. For custom bulk orders or rare‑target sourcing, trust CamelBio to bridge your concept‑to‑clinic journey.