Anti-Integrin alpha-V (CD51) Monoclonal Antibody for Flow Cytometry (Intracellular) - P06756

Core Product Specifications and Parameters

Biological Background: Integrin alpha-V Function and Localization

• Integrin alpha-V (ITGAV) is a type I transmembrane protein that pairs with beta subunits (e.g., ITGB3, ITGB5, ITGB6, ITGB8) to form functional vitronectin receptors and other RGD-binding integrins.
• The ITGAV:ITGB3 integrin acts as a co-receptor for several growth factors, including FGF1, FGF2, IGF1, IGF2, and IL1B, which is essential for their downstream signaling (PubMed:18441324, PubMed:28302677, PubMed:19578119, PubMed:28873464, PubMed:29030430). Related references: PMID:18441324 PMID:28302677 PMID:19578119 PMID:28873464 PMID:29030430
• Alpha-V integrins are required for the activation of latent TGF-β1: ITGAV:ITGB6 and ITGAV:ITGB8 bind to LAP and release active TGF-β1 through an RGD-dependent mechanism (PubMed:15184403, PubMed:22278742, PubMed:28117447). Related references: PMID:15184403 PMID:22278742 PMID:28117447
• ITGAV localizes predominantly to the plasma membrane and focal adhesion complexes, where it mediates cell attachment and signal transduction.
• ITGAV-containing integrins are exploited by numerous viruses for host cell entry, including adenovirus, coxsackievirus, herpesviruses, and HIV-1.
• The protein contains multiple domains, including several calcium-binding repeats, and undergoes extensive post-translational modifications such as glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• Because the immunogen is derived from the intracellular C-terminal region (aa 949-1048), this antibody is suited for intracellular flow cytometry. Permeabilization is required prior to staining.
• is a bright and photostable fluorophore; minimize light exposure during handling and storage to maintain signal intensity.
• Titrate the antibody concentration using your specific cell model to determine the optimal staining index for your assay.
• Include appropriate isotype controls and, when possible, validate staining in ITGAV knockout or knockdown cells to confirm specificity.
• For multicolor panels, ensure spectral compatibility and perform compensation using single-stained controls.

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