Anti-Integrin alpha-6/ITGA6 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P23229

Core Product Specifications and Parameters

Biological Background: Integrin alpha-6/ITGA6 Function and Localization

• Integrin alpha-6 (ITGA6), also designated CD49f or VLA-6, is a member of the integrin alpha chain family that heterodimerizes with beta-1 or beta-4 subunits to form laminin receptors.
• The ITGA6:ITGB1 complex functions as a laminin receptor on platelets and is present in oocytes, where it is implicated in sperm-egg fusion (By similarity).
• In epithelial cells, ITGA6:ITGB4 serves as a laminin receptor and is a key structural component of hemidesmosomes (By similarity).
• ITGA6:ITGB4 binds to neuregulin-1 (NRG1) via its EGF domain, an interaction essential for NRG1-ERBB signaling. Related references: PMID:20682778
• ITGA6:ITGB4 binds to insulin-like growth factors IGF1 and IGF2, interactions that are essential for IGF1 and IGF2 signaling, respectively. Related references: PMID:22351760 PMID:28873464
• The protein undergoes proteolytic cleavage on a pair of basic residues to yield the mature alpha-6 heavy and light chains; it is also palmitoylated and contains multiple disulfide bonds and calcium-binding sites.
• Multiple isoforms are generated by alternative splicing, with the ubiquitously expressed isoform containing the X1 cytoplasmic segment and a longer X1X2 form found in heart, kidney, placenta, and other tissues.
• Processed ITGA6 is expressed at low levels in normal prostate tissue and is upregulated in prostate cancer.
• ITGA6 localizes to the cell membrane as a single-pass type I membrane protein.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to an extracellular region (amino acids 505–663), making this antibody suitable for detecting cell-surface or native ITGA6 in non‑denaturing assays such as immunofluorescence.
• For western blotting, expect a major band at ~127 kDa; always include appropriate molecular‑weight markers and validate in your sample matrix due to potential glycosylation heterogeneity.
• In immunofluorescence and immunohistochemistry, staining should be membrane‑associated; co‑staining with a membrane marker is recommended to confirm specificity.
• ELISA performance may benefit from using recombinant integrin alpha‑6 protein or cell lysates as coating antigens; titrate antibody concentration for optimal signal‑to‑noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, connecting diagnostic developers with validated products like this anti‑ITGA6 monoclonal antibody. For your integrin, cell adhesion, or cancer biomarker projects, we offer one‑stop access to optimized antibody pairs, bulk ancillary reagents, and rare‑target sourcing, backed by technical consultation to streamline assay development.