Anti-Integrin alpha 5 (ITGA5/CD49e) Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (CD49e) is a transmembrane glycoprotein and member of the integrin alpha chain family. It pairs with integrin beta-1 (ITGB1) or beta-3 (ITGB3) to form functional heterodimers.
• The ITGA5:ITGB1 integrin serves as a receptor for fibronectin and fibrinogen, recognizing the RGD peptide sequence in these extracellular matrix proteins.
• As a receptor for fibrillin-1 (FBN1), ITGA5:ITGB1 mediates RGD-dependent cell adhesion, contributing to microfibril function. Related references: PMID:12807887 PMID:17158881
• ITGA5:ITGB1 binds to PLA2G2A at a non-classical site, inducing conformational changes that enhance ligand binding to the classical site. Related references: PMID:18635536 PMID:25398877
• The integrin alpha-5/beta-1 complex acts as a receptor for interleukin-1 beta (IL1B), and this interaction is essential for IL1B signaling. Related references: PMID:29030430
• When paired with ITGB3, ITGA5 functions as a receptor for soluble CD40 ligand (CD40LG), playing a role in CD40/CD40LG signaling. Related references: PMID:31331973
• In microbial infection contexts, ITGA5:ITGB1 acts as a receptor for human metapneumovirus, ITGA2:ITGB1 for parvovirus B19, and HIV-1 Tat protein interaction may enhance angiogenesis in Kaposi's sarcoma.
• The protein localizes to the cell membrane and focal adhesions, consistent with its role in cell adhesion and signaling.
• Post-translational modifications include cleavage on basic residues, N-glycosylation, phosphorylation, and the presence of disulfide bonds and calcium/metal-binding sites essential for function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 750–1000 in the C-terminal extracellular domain. For Western blot, expect a band near 115 kDa under reducing conditions; consider loading positive and negative controls.
• For immunofluorescence on paraffin sections (IF-P), optimize antigen retrieval and permeabilization steps. Membrane localization may require gentle permeabilization.
• In ELISA, use appropriate blocking (e.g., BSA) and validate with recombinant or native ITGA5 where possible.
• Due to cross-reactivity with mouse and rat, validate the antibody in the relevant species and tissue type if used for these model organisms.

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CamelBio offers a comprehensive portfolio of IVD raw materials, including high-quality antibodies targeting cell adhesion molecules such as integrin alpha-5. Our one-stop sourcing platform provides validated polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate diagnostic development. From rare target raw-material sourcing to full supply-chain support, CamelBio bridges the gap from concept to clinic.