Anti-Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb for WB, IHC-P, FC (intra), ELISA - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (ITGA5, CD49e, VLA-5) is the alpha subunit of the integrin alpha-5/beta-1 heterodimer, a classical fibronectin receptor that mediates cell adhesion via recognition of the RGD tripeptide sequence in extracellular matrix ligands (PubMed:33962943). Related references: PMID:33962943
• Beyond fibronectin, ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and for the inflammatory cytokine IL1B; the latter interaction is essential for IL1B signaling (PubMed:12807887, 17158881, 29030430). Related references: PMID:12807887 | PMID:17158881 | PMID:29030430
• ITGA5:ITGB1 also serves as a co-receptor for soluble CD40LG through its association with ITGB3, playing a role in CD40/CD40LG signaling (PubMed:31331973). Related references: PMID:31331973
• The protein localizes to the cell membrane and is enriched at focal adhesions, consistent with its role in cell-matrix attachment and mechanotransduction.
• ITGA5 is a single-pass type I membrane glycoprotein with a large extracellular domain, containing multiple calcium-binding repeats and a disulfide-linked heavy/light chain structure cleaved post-translationally at a pair of basic residues.
• In the context of infectious disease, ITGA5:ITGB1 functions as a host cell entry receptor for human metapneumovirus and, together with ITGA2:ITGB1, for human parvovirus B19.
• Additional annotations include a known 3D structure, membership in the reference proteome, and regulation by phosphorylation; at the protein level, ITGA5 is primarily detected in placenta.

Experimental Guidance and Technical Tips

• The immunogen targets the C‑terminal region (aa 950–1049), making this antibody suitable for intracellular flow cytometry (FC intra) and for detecting total ITGA5 in denaturing assays like western blot.
• For western blot, a band around 115–130 kDa is expected; protein migration may be influenced by glycosylation. Validate in the relevant sample system under reducing conditions.
• For immunohistochemistry (IHC-P), consider antigen retrieval and test on placenta or other positive control tissues, accompanied by appropriate negative controls.
• In ELISA or FC applications, pairing with an extracellular epitope antibody may be beneficial for native protein detection; otherwise, this reagent can serve as a capture or detection tool in sandwich assays.
• The antibody cross-reacts with mouse and rat ITGA5, enabling its use in rodent models. Epitope conservation should be confirmed by sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and research laboratories with a complete range of IVD raw materials. This validated rabbit monoclonal ITGA5 antibody supports studies in cell adhesion, extracellular matrix biology, and infectious disease mechanisms. Beyond single antibodies, we supply validated antibody pairs, customizable bulk formats, and rare‑target sourcing to streamline your assay development from concept to clinic.