Anti-Integrin alpha 5 (ITGA5/CD49e) Polyclonal Antibody for WB and ELISA - P08648

Core Product Specifications and Parameters

Biological Background: Integrin alpha-5 Function and Localization

• Integrin alpha-5 (UniProt P08648), also known as CD49e, fibronectin receptor subunit alpha, and VLA-5, is encoded by the ITGA5 gene. It is a single-pass type I membrane protein.
• ITGA5 pairs with integrin beta-1 to form the major fibronectin receptor, recognizing the R-G-D sequence and mediating cell adhesion, migration, and matrix assembly. Related references: PMID:33962943
• It also functions as a receptor for fibrinogen and fibrillin-1, contributing to diverse cell-matrix interactions and tissue integrity. Related references: PMID:18635536, PMID:12807887
• ITGA5:ITGB1 acts as a receptor for IL1B, a mechanism essential for interleukin-1 beta signaling. Related references: PMID:29030430
• ITGA5 can pair with beta-3 integrin to serve as a receptor for soluble CD40LG, which is required for CD40/CD40LG signaling. Related references: PMID:31331973
• The protein localizes to the cell membrane and is enriched at focal adhesions, consistent with its role in anchoring cells to the extracellular matrix.
• ITGA5 undergoes post-translational modifications including phosphorylation and glycosylation, and contains calcium-binding domains (UniProt keywords: Phosphoprotein, Glycoprotein, Calcium, Metal-binding).
• In addition to its physiological roles, ITGA5:ITGB1 serves as a receptor for several microbial pathogens, including human metapneumovirus and HIV-1 (via Tat protein).
• Tissue expression: detected at the protein level in placenta, with broader mRNA expression across many tissues.

Experimental Guidance and Technical Tips

• The immunogen spans the large extracellular domain (aa 42–995), likely generating polyclonal antibodies that recognize multiple epitopes. This broad reactivity can be advantageous for detection across species and in denaturing Western blot conditions.
• For Western blot, the predicted molecular weight is ~115 kDa (UniProt MW ~114.5 kDa). Validate antibody specificity by comparing signals in known positive and negative cell lines or tissues, and consider using a loading control.
• Given the polyclonal nature, the antibody may exhibit high sensitivity in ELISA. When developing sandwich immunoassays, pairing with a monoclonal detection antibody is recommended for improved specificity.
• Cross-reactivity with mouse enables this antibody to be used in mouse models; however, confirm reactivity in your specific experimental system, as epitope conservation may vary.
• Since ITGA5 is a cell adhesion molecule, consider that its expression can be modulated by the extracellular matrix context. For IHC/ICC applications (not listed here but common for integrins), additional validation would be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for cell adhesion and integrin research. For projects targeting ITGA5 or related integrins, we can source rare specificities, support assay development, and provide technical consultation tailored to your workflow. Explore our one-stop supply capabilities to accelerate your transition from biomarker discovery to clinical application.