Anti-Integrin alpha 5 (ITGA5/CD49e) Monoclonal Antibody () for FC - P08648

Core Product Specifications and Parameters

Biological Background: ITGA5/CD49e Function and Localization

• Integrin alpha-5 (ITGA5) is a type I transmembrane protein, also known as CD49e, VLA-5 alpha chain, and fibronectin receptor subunit alpha.
• ITGA5 pairs with integrin beta-1 (ITGB1) to form the fibronectin receptor, which binds to the RGD sequence in fibronectin and fibrinogen, mediating cell adhesion and signaling (PubMed:18635536, PubMed:25398877, PubMed:33962943).
  Related references:
  PMID:18635536 PMID:25398877 PMID:33962943
• ITGA5:ITGB1 also acts as a receptor for fibrillin-1 (FBN1) and mediates RGD-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
  Related references:
  PMID:12807887 PMID:17158881
• ITGA5:ITGB1 is involved in cytokine signaling, serving as a receptor for IL1B (via ITGA5:ITGB1) and soluble CD40LG (via ITGA5:ITGB3) (PubMed:29030430, PubMed:31331973).
  Related references:
  PMID:29030430 PMID:31331973
• In pathogen-host interactions, ITGA5:ITGB1 functions as a receptor for human metapneumovirus and HIV-1 Tat protein, facilitating viral entry or angiogenesis.
• Subcellularly, ITGA5 localizes to the cell membrane and cell junctions, particularly focal adhesions, where it connects the extracellular matrix to the actin cytoskeleton.
• Post-translational modifications: ITGA5 undergoes cleavage on pair of basic residues to yield heavy and light chains, and is N-glycosylated and phosphorylated, which may regulate its activity.
• According to UniProt, ITGA5 is expressed in placenta at the protein level, but may be present in other tissues as well.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 42-995, encompassing nearly the entire extracellular domain. This antibody is likely to recognize the native conformation, making it suitable for detection of cell-surface ITGA5 in flow cytometry.
• The antibody is directly conjugated to , a bright fluorophore, eliminating the need for secondary staining and reducing background. Optimize the antibody concentration by titration in the target cell line or sample of interest.
• Validate the reactivity in both human and mouse systems, as cross-reactivity is indicated. Include appropriate positive and negative controls (e.g., known integrin α5-expressing cells and isotype control) in your FC panel.
• For fixation and permeabilization steps, verify if the antibody recognizes the denatured epitope; if not, surface staining is recommended.
• As integrin α5 is involved in adhesion and signaling, consider using this antibody in conjunction with functional assays to correlate surface expression with activity.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted partner for IVD raw material procurement, CamelBio supplies diagnostic manufacturers, labs, and research institutes with high-quality antibodies and reagents. For integrin-related research and diagnostic assays, we offer validated monoclonal and polyclonal antibodies, optimized antibody pairs, and ancillary reagents to support your workflow. Whether you need bulk quantities or specialized targets, CamelBio streamlines your sourcing from discovery to clinical application.