Anti-Integrin alpha 4 (ITGA4/CD49d) Monoclonal Antibody for WB, IHC-P, ELISA - P13612

Core Product Specifications and Parameters

Biological Background: Integrin alpha-4 Function and Localization

• Integrin alpha-4 (ITGA4, CD49d) forms heterodimers with integrin beta-1 (ITGB1) to form VLA-4, and with integrin beta-7 to form LPAM-1, serving as receptors for fibronectin, VCAM1, and MADCAM1.
• Through VLA-4, ITGA4 recognizes the Q-I-D-S sequence in VCAM1, while alpha-4/beta-7 recognizes the L-D-T sequence in MADCAM1, mediating leukocyte rolling, firm adhesion, and transmigration.
• ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent signaling. Related references: PMID:23125415
• ITGA4:ITGB1 binds to PLA2G2A via a distinct site, inducing integrin conformational changes that enhance ligand binding. Related references: PMID:18635536, PMID:25398877
• In vascular smooth muscle cells, ITGA4:ITGB1 represses PRKCA-mediated L-type voltage-gated Ca2+ channel influx and ROCK-mediated calcium sensitivity via interaction with SVEP1, inhibiting vasocontraction. Related references: PMID:35802072
• ITGA4 is expressed on most leukocytes and is critical for immune cell trafficking; it is also expressed in vascular smooth muscle cells at the protein level.
• Subcellular localization is primarily at the plasma membrane as a single-pass type I membrane protein.
• Keywords include alternative splicing, cell adhesion, integrin, receptor, calcium, metal-binding, phosphoprotein, glycoprotein, and disulfide bond, highlighting its roles and regulation.

Experimental Guidance and Technical Tips

• Since the immunogen spans the C-terminal region (aa 933-1032), the antibody is expected to recognize the full-length integrin alpha-4 protein (~115 kDa) in WB. Validate band size with appropriate molecular weight markers and loading controls.
• For IHC-P, we suggest testing standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) and optimizing fixation protocols to preserve membrane epitopes.
• In ELISA, pair with an appropriate capture/detection antibody and confirm specificity using recombinant ITGA4 protein or cell lysates.
• Given cross-reactivity with mouse, this antibody can be considered for mouse tissue studies; however, we recommend pilot experiments to confirm reactivity in your specific model system.
• As integrins are glycosylated and may form disulfide bonds, consider reducing and non-reducing conditions in WB to assess potential dimerization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For integrin alpha-4 (ITGA4/CD49d) research, we provide reliable antibodies and related reagents to support your diagnostic assay development and cell adhesion studies. Contact us to explore customized sourcing solutions for rare targets and large-scale production needs.