Anti-Integrin alpha 3/CD49c Monoclonal Antibody for IHC-P, FC, ELISA - P26006

Core Product Specifications and Parameters

Biological Background: Integrin alpha-3 Function and Localization

• Integrin alpha-3, also known as CD49c, FRP-2, GAPB3, or VLA-3 subunit alpha, is a member of the integrin alpha chain family.
• It combines with integrin beta-1 to form the VLA-3 receptor, which binds to extracellular matrix proteins including fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4.
• This integrin heterodimer mediates cell adhesion, invadopodia formation, and matrix degradation, promoting cell invasion. It also facilitates endothelial cell migration via interaction with LGALS3 and CSPG4.
• In the context of microbial infection, ITGA3:ITGB1 may serve as a receptor for Rhizopus delemar CotH7 in alveolar epithelial cells, potentially involved in pulmonary mucormycosis.
• Subcellularly, integrin alpha-3 localizes to the cell membrane, as well as to specialized membrane structures such as invadopodia and filopodia.
• The protein undergoes proteolytic cleavage on basic residues, and is subject to post-translational modifications including glycosylation, palmitoylation, and phosphorylation.
• Two isoforms exist due to alternative splicing: isoform 1 is widely expressed, while isoform 2 is expressed in brain and heart, with distinct vascular distribution patterns.
• Keywords associated with ITGA3 include calcium, cell adhesion, cell membrane, integrin, receptor, and disease variants linked to epidermolysis bullosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 466-620 of human Integrin alpha 3, a region within the extracellular domain. This design may enable detection of total integrin alpha-3 protein.
• For IHC-P, consider optimizing antigen retrieval conditions and validate with known positive control tissues expressing ITGA3.
• For flow cytometry, confirm reactivity on cell lines with confirmed ITGA3 surface expression, and include appropriate isotype controls.
• For ELISA, evaluate pairing with a suitable capture antibody and assess sensitivity using recombinant integrin alpha-3 or cell lysates as antigen.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency; however, user validation in the intended assay system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic development from concept to clinic. This anti-integrin α3 antibody is part of our extensive portfolio for cell adhesion and cancer research, and we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development.