Anti-Integrin alpha 1/CD49a Rabbit PolymAb® for WB, ELISA - P56199 / Q3V3R4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-1 Function and Localization

• Integrin alpha-1 (CD49a, VLA-1) is a membrane glycoprotein that pairs with integrin beta-1 to form a heterodimeric receptor.
• Functions as a receptor for laminin and collagen, specifically recognizing the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
• Plays a role in anchorage-dependent, negative regulation of EGF-stimulated cell growth through interactions with the extracellular matrix.
• Contains several structural domains including calcium- and magnesium-binding sites essential for integrin activation and ligand binding.
• Undergoes post-translational modifications such as glycosylation and disulfide bond formation, contributing to its stability and function.

Experimental Guidance and Technical Tips

• The immunogen spans almost the entire extracellular domain (aa 29-1141), so the antibody likely recognizes multiple epitopes. Consider using it for Western blot detection of full-length and potentially truncated isoforms.
• Validate the antibody in relevant sample systems (human, mouse, rat) to confirm species cross-reactivity as indicated.
• For WB, the predicted molecular weight is ~130-131 kDa; ensure appropriate gel percentage and transfer conditions for high molecular weight proteins.
• For ELISA, perform checkerboard titrations to determine optimal coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing solution for IVD raw materials, including this high-quality anti-Integrin alpha 1/CD49a antibody. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your cell adhesion and cancer research applications. Contact us for customized supply of rare-target raw materials and technical consulting from concept to clinic.