Anti-Integrin alpha 1/CD49a Monoclonal Antibody for WB and IF - Q3V3R4

Core Product Specifications and Parameters

Biological Background: Integrin alpha 1 Function and Localization

• Integrin alpha-1 (CD49a) is a member of the integrin alpha chain family and pairs with integrin beta-1 to form the VLA-1 heterodimer.
• Functions as a major cell surface receptor for laminin and collagen, specifically recognizing the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
• Involved in anchorage-dependent negative regulation of EGF-stimulated cell growth, linking extracellular matrix to cell cycle control.
• Localized to the plasma membrane as a single-pass type I transmembrane protein.
• The protein contains multiple domains including FG-GAP repeats for ligand binding, and requires divalent cations (Ca2+, Mg2+) for activity.
• Post-translational modifications include glycosylation and disulfide bond formation, with a signal peptide directing it to the membrane.

Experimental Guidance and Technical Tips

• The immunogen covers a large region (aa 29-1141) of mouse Integrin alpha 1, which may enhance detection of native protein across applications.
• For Western blot, a band around 131 kDa is expected; validate in mouse or rat tissue lysates expressing Integrin alpha 1.
• In immunofluorescence (IF/ICC, IF-P), membrane localization should be observed; include appropriate controls.
• ELISA may be used for quantitative detection; consider pairing with a beta-1 integrin antibody if studying heterodimer formation.
• Cross-reactivity with rat has been confirmed; validate in the relevant sample system before use in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For researchers focused on integrin biology, we provide ready access to high-quality Integrin alpha 1/CD49a antibodies and can source rare target raw materials tailored to your assay development needs.