Anti-Integrin alpha 1 (ITGA1/CD49a) Rabbit Monoclonal Antibody for WB and ELISA - P56199

Core Product Specifications and Parameters

Biological Background: Integrin alpha-1 Function and Localization

• Integrin alpha-1 (ITGA1), also known as CD49a, VLA-1 alpha subunit, or laminin and collagen receptor, is a member of the integrin alpha chain family.
• It forms a heterodimer with integrin beta-1 to create the VLA-1 complex, which serves as a cell surface receptor for extracellular matrix proteins laminin and collagen.
• The receptor specifically recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen, mediating cell-matrix adhesion.
• ITGA1 is involved in anchorage-dependent negative regulation of EGF-stimulated cell growth, linking extracellular matrix signals to intracellular growth control.
• Subcellularly, integrin alpha-1 is a membrane-spanning glycoprotein, classified as a single-pass type I membrane protein.
• Key structural features include multiple disulfide bonds, calcium and magnesium binding sites, and a large extracellular domain with typical integrin repeats.
• Post-translational modifications include N-linked glycosylation, which is important for ligand binding and receptor function.
• Keywords from UniProt highlight its roles in calcium signaling, cell adhesion, metal ion binding, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen spans the large extracellular region (aa 29-1141), which may provide robust detection in both denaturing (WB) and native (ELISA) conditions.
• For Western blotting, the target protein has a molecular weight of approximately 130-131 kDa; a 6-8% SDS-PAGE gel and extended transfer times could improve resolution and transfer efficiency.
• In ELISA, consider using a direct or sandwich format and titrate the antibody concentration to optimize signal-to-noise ratio, as binding may depend on epitope accessibility.
• Since the antibody is reactive to human ITGA1, validate species cross-reactivity in samples from other species if needed, as no other species are listed.
• As a monoclonal antibody, it is expected to recognize a single epitope; ensure that the epitope is preserved under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, purified proteins, and ancillary reagents. For integrin and cell adhesion research, we offer optimized monoclonal antibodies like this anti-ITGA1/CD49a clone, along with custom sourcing for rare targets and technical support to accelerate your assay development. Partner with us to streamline your supply chain from concept to commercial production.