Anti-Insulin Receptor Rabbit Monoclonal Antibody for WB, ELISA - P06213

Core Product Specifications and Parameters

Biological Background: Insulin Receptor Function and Localization

• The insulin receptor is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin, including metabolic regulation and cell growth.
• Binding of insulin triggers autophosphorylation and recruitment of adaptor proteins (IRS1-4, SHC), activating two major pathways: the PI3K-AKT pathway responsible for metabolic effects (e.g., GLUT4 translocation) and the Ras-MAPK pathway controlling cell growth and differentiation.
• Two alternatively spliced isoforms (Long and Short) exist; Isoform Short shows higher affinity for IGFII. Hybrid receptors with IGF1R exhibit complex ligand-binding properties: Related references: PMID:12138094 PMID:16831875
• The receptor localizes to the cell membrane and is internalized into late endosomes and lysosomes upon activation.
• Predominantly expressed in classical insulin target tissues (liver, adipose, skeletal muscle) and also found in many other tissues; overexpressed in multiple tumor types, including breast, colon, and lung carcinomas.
• It is a glycoprotein and phosphoprotein, modified by disulfide bonds, proteolytic cleavage, and ubiquitin-like conjugation, which regulate its maturation and signaling.
• Genetic defects in INSR are linked to diabetes mellitus and severe insulin resistance syndromes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 900–1000 within the intracellular region of the insulin receptor; this epitope is present in both the full-length pro-receptor and the mature beta subunit, potentially enabling detection of both forms.
• For Western blotting, consider that the pro-receptor migrates at ~156 kDa, while the cleaved beta chain often appears around 95 kDa under reducing conditions. Validate detection sensitivity for both precursor and processed forms in your experimental system.
• For ELISA, use recombinant intracellular domain protein or lysates from INSR-expressing cells as antigen; confirm specificity with appropriate negative controls.
• The antibody cross-reacts with mouse and rat INSR, making it useful for translational studies; however, species-specific validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated IVD raw materials, including this anti-insulin receptor monoclonal antibody for research into insulin signaling, diabetes, and oncology. Our supply capabilities extend to optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and manufacturing needs. We are committed to accelerating your journey from concept to clinic with reliable, one-stop procurement solutions.