Anti-Insulin Receptor Polyclonal Antibody for WB, ELISA - P06213

Core Product Specifications and Parameters

Biological Background: Insulin Receptor Function and Localization

• Insulin receptor (IR) is a receptor tyrosine kinase that mediates the pleiotropic actions of insulin, including regulation of glucose uptake, cell growth, and differentiation.
• Upon insulin binding, IR phosphorylates insulin receptor substrates (IRS1-4) and SHC, activating the PI3K-AKT pathway for metabolic effects and the Ras-MAPK pathway for gene expression and cell growth.
• IR exists as two isoforms (Long and Short) from alternative splicing; isoform Short has higher affinity for IGF2, and hybrid receptors with IGF1R display distinct ligand sensitivities. Related references: PMID:12138094 PMID:16831875
• The receptor localizes to the cell membrane and, upon activation, internalizes to late endosomes and lysosomes.
• Post-translational modifications include autophosphorylation, glycosylation, and disulfide bond formation; it is a glycoprotein with tyrosine kinase activity.
• Expressed primarily in insulin-responsive tissues (liver, adipose, skeletal muscle) but also in many other tissues; overexpression is observed in various tumors.
• Mutations in INSR are associated with diabetes mellitus and severe insulin resistance syndromes.

Experimental Guidance and Technical Tips

• For Western blot, consider that the insulin receptor is processed into α (~135 kDa) and β (~95 kDa) subunits, leading to multiple bands depending on reducing conditions and sample preparation.
• Validate the antibody’s reactivity in your specific sample type (e.g., cell lysates, tissue extracts) using appropriate positive and negative controls, as the predicted molecular weight of the unprocessed precursor is ~156 kDa.
• For ELISA, titrate the antibody against recombinant insulin receptor or native receptor to optimize working concentrations, and include cross-reactivity validation for mouse and rat samples as indicated.
• Since the immunogen is a recombinant fragment (aa 475-570), the antibody may recognize both precursor and processed forms; confirm specificity via peptide competition or knockdown/knockout models if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research groups, providing one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This insulin receptor polyclonal antibody supports metabolic disease and insulin signaling research. With validated antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets, CamelBio ensures you get the right materials to accelerate your project.