Anti-Insulin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P01308

Core Product Specifications and Parameters

Biological Background: Insulin Function and Localization

• Insulin is a peptide hormone essential for regulating glucose homeostasis by lowering blood glucose concentrations.
• It enhances cellular uptake of monosaccharides, amino acids, and fatty acids.
• Insulin stimulates glycolysis, the pentose phosphate pathway, and glycogen synthesis, especially in the liver.
• Synthesized as preproinsulin, it undergoes proteolytic processing to yield mature insulin composed of A and B chains connected by disulfide bonds.
• The hormone is secreted from pancreatic beta-cells into the bloodstream in response to rising glucose levels.
• Subcellular localization: Extracellular, acting as a secreted signaling molecule.
• Tissue specificity: Predominantly expressed in pancreatic beta-cells at the protein level.
• Associated keywords: Hormone, diabetes mellitus, disulfide bond, signal peptide, alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions to break disulfide bonds that stabilize the hormone structure; validate detection in positive control lysates (e.g., pancreatic beta-cell lines or tissue).
• In immunofluorescence or immunohistochemistry, optimize antigen retrieval and fixation for pancreatic tissue to preserve insulin localization; co-staining with beta-cell markers may confirm specificity.
• For ELISA, evaluate pairing this antibody with a suitable detection antibody to develop a sensitive sandwich assay, testing across relevant biological matrices.
• Confirm cross-reactivity with mouse and rat insulin in your specific experimental system, as sequence homology may influence binding affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research teams with ready-to-use monoclonal antibodies optimized for metabolic and endocrine targets like insulin. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and customizable sourcing solutions to accelerate assay development for diabetes-related biomarkers. From rare target raw materials to production-scale supply, we help streamline your workflow from concept to clinic.