Anti-INSR/IGF1R Monoclonal Antibody for WB and ELISA - P06213 / P08069

Core Product Specifications and Parameters

Biological Background: INSR/IGF1R Function and Localization

• INSR encodes the insulin receptor, a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. This antibody also cross-reacts with the closely related insulin-like growth factor 1 receptor (IGF1R).
• Upon insulin binding, INSR phosphorylates insulin receptor substrates (IRS1-4), SHC, GAB1, and CBL, activating two main signaling cascades: the PI3K-AKT pathway (metabolic actions such as GLUT4 translocation and anti-apoptosis) and the Ras-MAPK pathway (cell growth and differentiation).
• INSR can form hybrid receptors with IGF1R, which bind IGF1, IGF2, and insulin with affinities that depend on the INSR isoform (Long vs. Short). These hybrids are found in multiple tissues and may contribute to signaling complexity. Related references: PMID:12138094 PMID:16831875
• Localized primarily at the cell membrane, the receptor is internalized into late endosomes and lysosomes following ligand stimulation, a key step for signal attenuation and receptor recycling.
• Predominantly expressed in classical insulin target tissues (liver, adipose, skeletal muscle), but also found in peripheral nerve, kidney, placenta, and various blood cells. Overexpression is reported in breast, colon, lung, ovary, and thyroid carcinomas.
• The receptor undergoes extensive post-translational modifications including glycosylation, disulfide bond formation, proteolytic cleavage into α/β subunits, and phosphorylation, with keywords highlighting ATP binding, kinase activity, and transmembrane signaling.

Experimental Guidance and Technical Tips

• For Western blot, consider lysates from insulin-responsive tissues (e.g., liver, muscle, adipose). The precursor form migrates at ~156 kDa, while the cleaved β‑subunit may appear near 95 kDa under reducing conditions. Validate in the relevant sample system.
• In ELISA, the antibody may be used for capture or detection. Because the immunogen lies within the C‑terminal region of the β‑subunit (residues 1100‑1200), it can recognize both full‑length receptor and the processed β‑chain. Test compatibility with detection antibodies.
• The antibody cross‑reacts with human, mouse, and rat INSR/IGF1R. Confirm specificity for the intended target using appropriate controls (e.g., knockdown or knockout cell lysates) to distinguish INSR from IGF1R signals.
• As a rabbit monoclonal, this reagent offers lot‑to‑lot consistency. For quantitative applications, include proper loading controls and consider that post‑translational modifications may affect migration or epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides more than antibodies; we offer one-stop access to IVD raw materials, technical expertise, and consulting—guiding projects from concept to clinic. This INSR/IGF1R antibody is positioned for research in metabolic signaling and oncology. For assay development, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Contact CamelBio to explore integrated support tailored to your diagnostic needs.