Anti-INSIG2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y5U4

Core Product Specifications and Parameters

Biological Background: INSIG2 Function and Localization

• INSIG2 is an oxysterol-binding protein that mediates feedback control of cholesterol synthesis by regulating endoplasmic reticulum (ER)-to-Golgi transport of SCAP and degradation of HMGCR. Related references: PMID:12242332 PMID:16606821 PMID:32322062
• It acts as a negative regulator of sterol regulatory element-binding protein (SREBP) processing: in the presence of oxysterols, INSIG2 binds SCAP, retaining the SCAP-SREBP complex in the ER and preventing proteolytic activation of SREBF1/SREBP1 and SREBF2/SREBP2. Related references: PMID:32322062
• INSIG2 promotes sterol-induced ubiquitin-mediated ER-associated degradation (ERAD) of HMGCR by recruiting the ubiquitin ligase RNF139. Related references: PMID:16606821 PMID:22143767
• Oxysterol binding (e.g., 22-, 24-, 25-, and 27-hydroxycholesterol) modulates its interaction with SCAP and is critical for its regulatory functions. Related references: PMID:17428920 PMID:26160948 PMID:32322062
• INSIG2 is an integral membrane protein localized to the endoplasmic reticulum membrane.
• It participates in cholesterol and lipid metabolism pathways, as highlighted by UniProt keywords: Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitin conjugation (UniProt keywords: Phosphoprotein, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within the N-terminal region (aa 50–150) of human INSIG2; this antibody likely recognizes linear epitopes and can be used under denaturing conditions.
• For Western blot, a reduced/denatured sample preparation is typically suitable; expect a band at ~25 kDa. Validate detection in your specific cell or tissue lysate with positive and negative controls.
• In IHC‑P, optimize antigen retrieval and antibody dilution using tissue known to express INSIG2 (e.g., cholesterol‑metabolizing tissues). Include a blocking peptide control to confirm specificity.
• For ELISA, pair this polyclonal antibody with a matched detection antibody if quantitative sandwich formats are required; cross‑reactivity with mouse and rat allows multi‑species sample analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a seamless sourcing experience for IVD raw materials, from concept to clinic. This anti‑INSIG2 polyclonal antibody supports cholesterol and lipid metabolism research, and is part of a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For rare‑target or custom‑development needs, CamelBio bridges the gap between your assay requirements and reliable supply.