Anti-INPP5J Polyclonal Antibody for WB, IF/ICC, ELISA - Q15735

Core Product Specifications and Parameters

Biological Background: INPP5J Function and Localization

• INPP5J, also known as PIPP, INPP5, PIB5PA, is a phosphatidylinositol 4,5-bisphosphate 5-phosphatase A, a member of the inositol polyphosphate 5-phosphatase family.
• It catalyzes the dephosphorylation of inositol 1,4,5-trisphosphate (IP3) to inositol 1,4-bisphosphate (IP2) and converts phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 4-phosphate (PIP), thus modulating phosphoinositide signaling.
• The enzyme also acts on inositol 1,3,4,5-tetrakisphosphate (IP4) in vitro, converting it to inositol 1,3,4-trisphosphate, and may regulate the function of phosphoinositide-binding proteins at membrane ruffles.
• INPP5J is primarily localized in the cytoplasm, consistent with its role in soluble inositol phosphate metabolism.
• The protein undergoes alternative splicing, phosphorylation, and methylation, and contains SH3-binding motifs, suggesting regulatory interactions with signaling complexes.
• It requires magnesium as a metal cofactor for catalytic activity, characteristic of the 5-phosphatase family.
• INPP5J has a predicted molecular weight of approximately 107 kDa, as annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 60-360) of human INPP5J, which is conserved across human, mouse, and rat, supporting cross-reactivity predictions. Consider sequence alignment to verify in your specific model.
• For Western blot (WB), INPP5J has a predicted molecular weight of ~107 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC experiments, cytoplasmic localization is expected; optimize fixation and permeabilization protocols to preserve native protein distribution.
• For ELISA, the polyclonal nature may provide robust detection; include appropriate controls and standard curves for quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials for phosphoinositide signaling research, including validated antibodies like this anti-INPP5J polyclonal antibody. As your one-stop partner, we supply optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic.