Anti-INPP5E Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NRR6

Core Product Specifications and Parameters

Biological Background: INPP5E Function and Localization

• INPP5E, also known as phosphatidylinositol polyphosphate 5-phosphatase type IV, 72 kDa inositol polyphosphate 5-phosphatase, or pharbin, is encoded by the INPP5E gene (GeneID: 56623).
• It specifically hydrolyzes the 5-phosphate from phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), being selective for lipid substrates and inactive toward water-soluble inositol phosphates. Related references: PMID:10764818
• INPP5E can also remove the 5-phosphate from 1D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3). Related references: PMID:40969890
• The protein plays a critical role in primary cilium function by regulating ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability.
• Subcellular localization includes the cytoplasm, cytoskeleton, cilium axoneme, Golgi apparatus, cell membrane, ruffles, and nucleus.
• It is expressed in a tissue‑specific manner, with detection in brain, heart, pancreas, testis, and spleen.
• Mutations in INPP5E are associated with ciliopathies such as Joubert syndrome, and the protein is classified under keywords encompassing disease variant, intellectual disability, and obesity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 510‑630) of human INPP5E; thus, the antibody may recognize all isoforms sharing this domain. Verify isoform-specific expression in your target samples.
• For Western blot, the expected molecular weight is approximately 70 kDa. Use appropriate loading controls and consider potential post‑translational modifications that may shift mobility.
• In immunofluorescence (IF/ICC), localization to the primary cilium and Golgi apparatus may require optimized fixation/permeabilization protocols. Co‑staining with cilium markers (e.g., acetylated tubulin) is recommended.
• This antibody is polyclonal; batch‑to‑batch variability is inherent. Validate specificity in knockout or knockdown controls where possible.
• When using ELISA, include suitable negative controls to assess background signal, and optimize coating antigen concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide one‑stop access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. This anti‑INPP5E polyclonal antibody expands your toolkit for investigating phosphoinositide signaling and ciliopathy‑related pathways. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets to streamline your assay development workflows.