Anti-INPP5A Polyclonal Antibody for Western Blot and ELISA - Q14642

Core Product Specifications and Parameters

Biological Background: Inositol polyphosphate-5-phosphatase A Function and Localization

• INPP5A specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate (IP3) to inositol 1,4-bisphosphate (IP2), and inositol 1,3,4,5-tetrasphosphate (IP4) to inositol 1,3,4-trisphosphate (IP3). Related references: PMID:40969890, PMID:8013665, PMID:8626616
• The enzyme plays a crucial role in the survival of cerebellar Purkinje cells (By similarity).
• Predominantly expressed in heart, brain, and skeletal muscle, with high levels in brain Purkinje cells. Related references: PMID:8006039, PMID:8013665
• Localizes to the cell membrane and dendritic projections (Cell projection, dendrite).
• INPP5A is a magnesium-dependent hydrolase and lipoprotein, subject to prenylation.
• The human INPP5A gene encodes a protein with a predicted molecular weight of 47.8 kDa, migrating at approximately 48 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10–200 of human INPP5A, covering the N-terminal region; researchers may consider epitope accessibility in different sample preparations.
• For Western blotting, a band around 48 kDa is expected; validate expression in heart, brain, or skeletal muscle lysates where INPP5A is abundant.
• The antibody cross-reacts with mouse and rat orthologs; verify cross-reactivity in the relevant species’ cell or tissue samples prior to critical experiments.
• For ELISA applications, titrate the antibody using recombinant INPP5A or peptide standards to establish optimal working concentrations.
• Consider appropriate positive and negative controls, such as lysates from tissues with known high versus low INPP5A expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated anti-INPP5A antibodies for inositol phosphate signaling studies. Our portfolio offers comprehensive antibody solutions—from monoclonal and polyclonal antibodies to bulk ancillary reagents—optimized for consistent assay performance. As your one-stop sourcing partner, we also facilitate procurement of rare-target raw materials and provide technical consultation to support your product development from concept to clinic.