Anti-INPP4B Rabbit Polyclonal Antibody for WB and ELISA - O15327

Core Product Specifications and Parameters

Biological Background: INPP4B Function and Localization

• INPP4B encodes inositol polyphosphate 4-phosphatase type II, also known as type II inositol 3,4-bisphosphate 4-phosphatase.
• Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate, and inositol 3,4-trisphosphate. Related references: PMID:24070612, PMID:24591580
• Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles. Related references: PMID:24591580
• The lipid phosphatase activity is critical for tumor suppressor function; it antagonizes the PI3K-AKT/PKB signaling pathway, thereby modulating cell cycle progression and cell survival. Related references: PMID:19647222, PMID:24070612
• Widely expressed with highest levels in skeletal muscle and heart.
• Keywords: alternative splicing, hydrolase, lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 380–600, a region that may be conserved across species, supporting the observed cross-reactivity with human, mouse, and rat.
• For WB, consider testing whole-cell lysates from tissues with known INPP4B expression (e.g., skeletal muscle or heart) and validate against appropriate controls.
• In ELISA applications, confirm coating antigen compatibility with the antibody’s specificity, as the polyclonal nature may recognize multiple epitopes within the immunogen region.
• To distinguish specific bands from background, include a negative control (e.g., lysate from cells with low or silenced INPP4B expression) and optimize blocking conditions.
• Since INPP4B is involved in PI3K-AKT signaling, verify antibody performance in relevant cell models stimulated or inhibited for pathway activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies for key signaling targets like INPP4B. Our supply capabilities cover bulk ancillary reagents and rare target raw-material sourcing to support your PI3K pathway research and assay development. Whether you need customized antibody production or reliable, batch-tested reagents, CamelBio bridges the gap from concept to clinic with a streamlined procurement experience.