Anti-INPP4A Polyclonal Antibody for WB, ELISA - Q96PE3

Core Product Specifications and Parameters

Biological Background: INPP4A Function and Localization

• INPP4A encodes type I inositol polyphosphate-4-phosphatase, which catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2), as well as inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate.
  Related references:
  PMID:15716355
  PMID:20463662

• By dephosphorylating phosphoinositides, INPP4A antagonizes the PI3K-AKT/PKB signaling pathway, thereby modulating cell cycle progression and cell survival.
  Related references:
  PMID:30071275

• The protein may protect neurons from excitotoxic cell death by regulating the synaptic localization of cell surface N-methyl-D-aspartate-type glutamate receptors (NMDARs) and NMDAR-mediated excitatory postsynaptic currents.

• INPP4A localizes to multiple subcellular compartments, including early endosome and recycling endosome membranes, the cell membrane, nucleus, cytoplasm, and postsynaptic density.

• Alternative splicing generates two isoforms: isoform 1 is expressed in platelets, MEG-01 megakaryocytes, and Jurkat T-cells, while isoform 2 is predominantly expressed in the brain.

• The gene is associated with intellectual disability, as indicated by the disease variant keyword, highlighting its importance in neurodevelopment.

• Other keywords include hydrolase, lipid metabolism, phosphoprotein, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–250 of human INPP4A) is conserved across human, mouse, and rat, supporting the listed cross-reactivity; validate detection in your species of interest.
• For Western blotting, expect a band near 110 kDa in lysates from tissues or cells expressing INPP4A; use appropriate positive (e.g., brain lysate) and negative controls.
• In ELISA, the antibody may be used to capture or detect recombinant or endogenous INPP4A; optimize coating concentration and secondary detection conditions.
• Pre-incubation with the immunogen can serve as a specificity control to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement for diagnostic manufacturers and research labs by offering validated antibodies like this anti-INPP4A polyclonal antibody. We supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets in phosphoinositide signaling and neurobiology. Partner with us to streamline your assay development from concept to clinic.