Anti-iNOS Rabbit Polyclonal Antibody for WB and ELISA - P35228

Core Product Specifications and Parameters

Biological Background: iNOS Function and Localization

• Nitric oxide synthase, inducible (iNOS), also known as NOS2A, HEP-NOS, or NOS type II, is a calcium/calmodulin-dependent enzyme that catalyzes the production of nitric oxide (NO) from L-arginine, with diverse signaling functions. Related references: PMID:7504305, PMID:7531687, PMID:7544004
• In macrophages, iNOS-derived NO mediates tumoricidal and bactericidal actions, and the enzyme also exhibits nitrosylase activity, catalyzing cysteine S-nitrosylation of cytoplasmic targets like PTGS2/COX2. Related references: PMID:25417112
• As part of the iNOS-S100A8/9 transnitrosylase complex, iNOS facilitates stimulus-dependent S-nitrosylation of GAPDH at 'Cys-247', influencing GAIT complex activity and modulating targets such as ANXA5, EZR, MSN, and VIM. Related references: PMID:25417112
• iNOS is involved in inflammation, enhancing the synthesis of pro-inflammatory mediators like IL6 and IL8. Related references: PMID:19688109
• Subcellularly, iNOS localizes to the cytoplasm and cytosol, consistent with its enzymatic function.
• Tissue expression is reported in the liver, retina, bone cells, airway epithelial cells of the lung, and chondrocytes; it is not expressed in platelets. Related references: PMID:7504305
• iNOS belongs to the NOS family and contains multiple cofactor-binding domains, including FAD, FMN, heme, and NADP, with regulatory calcium/calmodulin-binding and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 974–1143 of human iNOS (NP_000616.3), a C-terminal region; this polyclonal antibody may recognize both full-length and truncated forms, but validation in the target sample system is advised.
• For Western blot, a band around 131 kDa is expected; positive controls can include lysates from IFN-γ/LPS-stimulated RAW 264.7 (mouse) or THP-1 (human) macrophage cells.
• Cross-reactivity with mouse iNOS is reported; for other species, test in appropriate models before routine use.
• In ELISA, the antibody can be used for detection or capture; optimization of coating concentration and blocking conditions is recommended for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive pipeline of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-iNOS antibody, and ancillary reagents. For projects involving inflammation, macrophage biology, or NO signaling, CamelBio can source rare-target materials and offer technical consulting to streamline your assay development.