Anti-iNOS Rabbit Monoclonal Antibody for WB - P35228

Core Product Specifications and Parameters

Biological Background: iNOS Function and Localization

• Nitric oxide synthase, inducible (iNOS), encoded by the NOS2 gene, is a 131 kDa enzyme that produces nitric oxide (NO), a versatile signaling molecule with roles in immune response, vasodilation, and neurotransmission. Related references: PMID:7504305, PMID:7531687, PMID:7544004
• In macrophages, iNOS-derived NO mediates tumoricidal and bactericidal activities, contributing to the innate immune defense.
• iNOS also functions as a peptidyl-cysteine S-nitrosylase, mediating cysteine S-nitrosylation of cytoplasmic targets such as PTGS2/COX2 (by similarity), and participates in the iNOS-S100A8/9 transnitrosylase complex that selectively S-nitrosylates GAPDH, ANXA5, EZR, MSN, and VIM in an inflammatory stimulus-dependent manner. Related references: PMID:25417112
• iNOS is involved in inflammatory processes, enhancing the synthesis of pro-inflammatory mediators like IL-6 and IL-8. Related references: PMID:19688109
• Subcellularly, iNOS localizes to the cytoplasm and cytosol.
• Tissue expression includes the liver, retina, bone cells, airway epithelial cells of the lung, and chondrocytes; it is absent from platelets. Related references: PMID:7504305
• Key structural and functional features include binding sites for calmodulin, heme, FAD, FMN, NADP, and metal ions (zinc, iron), and the protein is subject to alternative splicing, phosphorylation, and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2-200 of human iNOS, an N-terminal region; this design may provide specificity for iNOS detection in various assays.
• For Western blotting (WB), the expected molecular weight is ~131 kDa; ensure adequate transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), iNOS typically shows cytoplasmic and cytosolic staining; optimize cell fixation and permeabilization for your model system.
• For ELISA-based applications, consider pairing this antibody with a suitable detection antibody if constructing a sandwich format.
• This antibody demonstrates cross-reactivity with mouse and rat iNOS; validate specificity and titrate in the relevant species-specific sample context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. For nitric oxide signaling and iNOS-related inflammatory research, we offer a portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our capabilities also include sourcing rare target raw materials and providing technical consultation, streamlining your assay development and manufacturing supply chain.