Anti-iNOS Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P29477

Core Product Specifications and Parameters

Biological Background: iNOS Function and Localization

• Produces nitric oxide (NO), a messenger molecule with diverse functions. In macrophages, NO mediates tumoricidal and bactericidal actions. Also possesses nitrosylase activity, catalyzing cysteine S-nitrosylation of cytoplasmic proteins such as PTGS2/COX2. Related references: PMID:7503239, PMID:16373578
• As component of the iNOS-S100A8/9 transnitrosylase complex, is involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH, regulating the GAIT complex activity, and potentially modifies ANXA5, EZR, MSN, and VIM (By similarity).
• Involved in inflammation, enhancing the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity).
• Localizes predominantly to the cytoplasm and cytosol.
• Expressed mainly in macrophages.
• Keywords: Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP, Oxidoreductase, Phosphoprotein, Ubl conjugation, Zinc, indicating a multi-cofactor enzyme subject to post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-200) of mouse iNOS, which may be responsible for the observed cross-reactivity with human and rat iNOS.
• For Western blot, expect a band at approximately 131 kDa; optimize gel percentage and transfer conditions accordingly.
• In immunofluorescence/ICC, validate fixation and permeabilization protocols as iNOS is a cytosolic protein.
• For ELISA, consider using the recombinant iNOS protein as a standard or capture reagent.
• Cross-reactivity with human and rat is reported; confirm sequence homology for species-specific applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and researchers, providing one-stop access to high-quality IVD raw materials and technical support. This anti-iNOS antibody supports studies in nitric oxide signaling, inflammation, and macrophage biology. Explore CamelBio's portfolio for validated antibody pairs, ancillary reagents, and customized sourcing solutions to accelerate your diagnostic assay development.