Anti-iNOS Monoclonal Antibody for WB, IF/ICC, ELISA - P35228

Core Product Specifications and Parameters

Biological Background: iNOS Function and Localization

• Encoded by the NOS2 gene (also known as iNOS, NOS2A, HEP-NOS), this inducible nitric oxide synthase is a key enzyme in the NOS family, expressed in multiple tissues including liver, retina, bone, and airway epithelium.
• Produces nitric oxide (NO), a versatile messenger molecule with roles ranging from vasodilation to immune defense (PubMed:7504305, PubMed:7531687, PubMed:7544004). Related references: PMID:7504305 PMID:7531687 PMID:7544004
• In macrophages, NO mediates tumoricidal and bactericidal actions, contributing to innate immunity.
• Functions as a peptidyl-cysteine S-nitrosylase, mediating S-nitrosylation of cytoplasmic targets such as PTGS2/COX2 (by similarity) and GAPDH (PubMed:25417112). As part of the iNOS-S100A8/9 transnitrosylase complex, it selectively S-nitrosylates GAPDH at Cys-247, modulating GAIT complex activity and inflammatory responses. Related references: PMID:25417112
• Enhances the synthesis of pro-inflammatory mediators IL6 and IL8, further amplifying inflammatory cascades (PubMed:19688109). Related references: PMID:19688109
• Localized predominantly in the cytoplasm/cytosol, where it interacts with calmodulin and other cofactors for enzymatic activity.
• Subject to post-translational modifications including phosphorylation and ubiquitination, and requires multiple cofactors such as heme, FAD, FMN, NADP, and zinc for its catalytic function.

Experimental Guidance and Technical Tips

• The predicted molecular weight is 131 kDa; for Western blot, a band near this size is expected. Use reducing conditions and validate with a relevant positive control (e.g., LPS/IFN-γ stimulated RAW264.7 mouse macrophages).
• For immunofluorescence, fix cells gently and consider co-staining with a cytoplasmic marker. Verify expression in your system, as iNOS is inducible.
• In ELISA applications, ensure the capture/detection antibody pair is compatible; the monoclonal nature of this antibody may provide consistent specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories worldwide with a comprehensive portfolio of IVD raw materials. This high-quality iNOS monoclonal antibody is suitable for your inflammation and nitric oxide research needs. We offer sourcing for validated antibody pairs, bulk reagents, and technical consultation to support your workflow from concept to clinic.