Anti-INHBB Rabbit Polyclonal Antibody for WB, ELISA - P09529

Core Product Specifications and Parameters

Biological Background: INHBB Function and Localization

• INHBB encodes the inhibin beta B chain, a member of the TGF-beta superfamily, which dimerizes with the inhibin alpha chain to form inhibin B or with another beta-B chain to form activin B. These complexes exhibit opposing effects on follicle-stimulating hormone (FSH) secretion.
• Activin B (beta-B:beta-B dimer) signals through type I receptor ACVR1C and type II receptors such as ACVR2A or BMPR2, leading to phosphorylation of SMAD2 and SMAD3, which then complex with SMAD4 to regulate gene transcription. This pathway is central to embryonic development, stem cell maintenance, hematopoiesis, cell proliferation, and wound healing.
  Related references:
  PMID:15196700
  PMID:35643319
• Activin B plays a critical role in the induction of hepcidin during inflammation by activating ACVR1C and subsequent phosphorylation of SMAD1/5/8. This links INHBB to iron homeostasis and inflammatory responses.
  Related references:
  PMID:22611157
• Inhibin B (alpha:beta-B dimer) acts as a competitive antagonist of activin signaling, potently suppressing FSH release from the anterior pituitary, thereby regulating gonadal function and reproductive homeostasis.
• The INHBB precursor contains a signal peptide and undergoes proteolytic cleavage to yield mature secreted forms. The full-length proprotein has a calculated molecular weight of approximately 45 kDa, consistent with the detected band.
• Subcellular location: secreted. Key post-translational modifications include disulfide bond formation and glycosylation, characteristic of secreted glycoprotein hormones.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 293-407 near the C-terminus of the INHBB precursor, which may provide specificity for the proprotein and mature forms. For Western blot, a band at approximately 45 kDa is expected; additional bands representing processed subunits might be observed. Validate detection in relevant cell lysates or tissue extracts.
• For ELISA applications, titrate the antibody and validate using recombinant protein standards or biological samples as per protocol optimization.
• As a polyclonal antibody, it may recognize multiple epitopes, offering robust signal but potential cross-reactivity with other TGF-beta family members. Confirmation by antigen competition or isoform-specific controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to diagnostic developers and research labs. For your INHBB‑focused projects in reproductive biology, stem cell research, or inflammation, we can source bulk quantities of this polyclonal antibody along with technical support to accelerate assay development and validation. With a comprehensive portfolio and end‑to‑end sourcing capability, CamelBio bridges the gap from concept to clinic.