Anti-ING4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UNL4

Core Product Specifications and Parameters

Biological Background: ING4 Function and Localization

• ING4 is a member of the inhibitor of growth (ING) family of tumor suppressors, encoded by the ING4 gene; alternative splicing generates multiple isoforms.
• As a component of HBO1 complexes, ING4 specifically mediates acetylation of histone H3 at Lys-14 (H3K14ac) and may play a role in DNA replication. Related references: PMID:16387653
• ING4 can suppress tumor progression by modulating transcriptional outputs of signaling pathways that control cell proliferation. Related references: PMID:15251430, PMID:15528276
• Complexed with RELA, ING4 represses RELA/NFKB3 target genes to suppress brain tumor angiogenesis, and also specifically suppresses loss of contact inhibition induced by activated oncogenes like MYC. Related references: PMID:15029197
• ING4 represses hypoxia-inducible factor (HIF) activity through interaction with HIF prolyl hydroxylase 2 (EGLN1). Related references: PMID:15897452
• By similarity, ING4 can enhance apoptosis triggered by serum starvation in mammary epithelial cell lines.
• Localized primarily in the nucleus, ING4 functions as a chromatin regulator and contains conserved domains such as a zinc-finger and coiled-coil region, indicative of DNA/RNA binding and protein interaction capabilities.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human ING4 (aa 1–249), including the conserved N-terminal region, which likely ensures recognition of multiple splice isoforms and cross-reactivity with mouse ING4.
• For western blot (WB), a band near 29 kDa is expected; validate in the relevant cell or tissue lysate with appropriate loading controls.
• IHC-P and ELISA protocols should be individually optimized; consider using known positive and negative control samples and follow standard antigen retrieval and blocking steps for IHC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to a broad portfolio of IVD raw materials, including validated polyclonal antibodies such as this anti-ING4 antibody targeting chromatin and tumor suppressor pathways. Our comprehensive sourcing services cover customized antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material acquisition for rare targets. Partner with us to bridge your assay development from concept to clinic with reliable supply and technical expertise.