Anti-ING1 Rabbit Polyclonal Antibody for WB and ELISA - Q9UK53

Core Product Specifications and Parameters

Biological Background: ING1 Function and Localization

• Identified as inhibitor of growth protein 1 (ING1), a member of the ING tumor suppressor family, with multiple isoforms (p33, p47, etc.) produced by alternative splicing.
• Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation, thereby acting as a tumor suppressor.
• Localizes to the nucleus, consistent with its role in transcriptional regulation and cell cycle control.
• Exhibits tissue-specific expression of isoforms: isoform 2 is widely expressed, while isoforms 3 and 4 show restricted patterns in tissues such as testis, liver, and brain.
• Contains a zinc finger domain, implicating metal-binding capability, and is subject to post-translational modifications including isopeptide bond formation and ubiquitin-like conjugation.
• Involved in cell cycle regulation, with potential disease-associated variants, reinforcing its significance in cancer biology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (amino acids 80-180) of human ING1. This region may be conserved across isoforms; validation of isoform specificity is recommended.
• For Western blot, the predicted molecular weight of the canonical isoform is 47 kDa, but alternative isoforms may appear at different sizes. Consider using a positive control such as HeLa or other human cell lines with known ING1 expression.
• For ELISA applications, optimize antibody concentration and assay conditions for your specific format; the antibody can be explored as either capture or detection reagent.
• Cross-reactivity is indicated for human; for use with other species, align the immunogen peptide sequence to the target protein sequence to assess potential reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. This anti-ING1 polyclonal antibody is available for your cancer research and diagnostic assay development, alongside validated antibody pairs and bulk reagents. Our sourcing capabilities also extend to rare targets and ancillary materials, supporting your workflow from concept to clinic.