Anti-Indolethylamine N-methyltransferase (TEMT) Rabbit Monoclonal Antibody for WB, ELISA - O95050

Core Product Specifications and Parameters

Biological Background: Indolethylamine N-methyltransferase (TEMT) Function and Localization

• Protein identity and isoforms: Indolethylamine N-methyltransferase (INMT), also known as thioether S-methyltransferase (TEMT) and aromatic alkylamine N-methyltransferase, catalyzes the N-methylation of tryptamine and related compounds, as well as the S-methylation of thioethers. Alternative splicing generates multiple isoforms.
• Core nucleosome/chromatin function: Not applicable; this enzyme is a methyltransferase involved in small molecule metabolism rather than chromatin modification.
• Gene family members: INMT belongs to the methyltransferase superfamily and utilizes S-adenosyl-L-methionine as a methyl donor.
• Subcellular localization: Predominantly localized in the cytoplasm.
• Tissue distribution: Widely expressed, with highest levels in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node, and trachea. Very low levels in adult and fetal kidney and liver, spleen, thymus, ovary, colon, and bone marrow. Not detected in peripheral blood leukocytes and brain.
• Key post-translational modifications and keywords: Features include a resolved 3D structure; involved in detoxification, methylation, and selenium compound metabolism.
• Antimicrobial roles: No known antimicrobial function; primarily involved in xenobiotic detoxification and endogenous amine metabolism.

Experimental Guidance and Technical Tips

• For Western blotting, use reducing conditions and expect a band near 29 kDa. Validate the antibody in relevant tissue lysates (e.g., thyroid or lung) or cell lines expressing INMT.
• For ELISA, consider pairing with a recombinant TEMT protein standard to establish assay sensitivity and specificity.
• Given cross-reactivity with mouse, this antibody may be suitable for mouse model studies; however, verify performance in the specific mouse tissue of interest.
• This monoclonal antibody targets the N-terminal region (aa 1–150) and may recognize multiple isoforms; confirm specificity by knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This TEMT antibody is available for diagnostic manufacturers and researchers studying methylation and detoxification pathways. We offer bulk quantities, validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development and rare target sourcing needs.