Anti-INCENP Polyclonal Antibody for WB and ELISA - Q9NQS7

Core Product Specifications and Parameters

Biological Background: INCENP Function and Localization

• INCENP is a core scaffold component of the chromosomal passenger complex (CPC), a key mitotic regulator that ensures correct chromosome alignment and segregation.
  Related references: PMID:12925766, PMID:15316025, PMID:27332895
• The N‑terminus of INCENP binds BIRC5/survivin and CDCA8/borealin to tether the CPC to the inner centromere, while the C‑terminus interacts with AURKB or AURKC.
  Related references: PMID:12925766, PMID:15316025, PMID:27332895
• A central SAH domain mediates microtubule binding, directing AURKB/C kinase activity toward substrates on dynamic microtubules.
  Related references: PMID:12925766, PMID:15316025, PMID:27332895
• INCENP activates AURKB and AURKC, and the flexible SAH domain is proposed to allow the complex to follow substrates on microtubules while remaining anchored at the inner centromere.
  Related references: PMID:27332895
• INCENP is required for the localization of CBX5 to mitotic centromeres.
  Related references: PMID:21346195
• INCENP controls the kinetochore localization of BUB1, contributing to the mitotic checkpoint.
  Related references: PMID:16760428
• Subcellularly, INCENP localizes to the nucleus, centromere, kinetochore, spindle, and midbody, consistent with its roles in chromosome segregation and cytokinesis.
• INCENP undergoes phosphorylation and alternative splicing, generating multiple protein isoforms that may fine‑tune CPC functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 785‑914) of human INCENP, which includes part of the IN‑box domain; this antibody may recognize full‑length INCENP and potentially C‑terminal fragments.
• For Western blot, consider a standard SDS‑PAGE with 8‑10% gel, transfer to PVDF, and blocking with 5% non‑fat milk. Detection can be performed using a standard ECL reagent; validate in your specific lysate system.
• For ELISA, the recombinant immunogen or a peptide from the target region may serve as a positive control; note that cross‑reactivity with rat has been observed in supplier tests.
• As this is a polyclonal antibody, lot‑to‑lot consistency should be verified for critical quantitative assays, and inclusion of an appropriate loading control is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and IVD developers streamlined access to a broad portfolio of high‑quality raw materials, including validated antibodies for cell cycle and mitosis research. This anti‑INCENP polyclonal antibody exemplifies our commitment to providing reliable immunoreagents for critical applications; beyond catalog products, we source customized antibody pairs, bulk ancillary reagents, and rare‑target raw materials to support your entire development workflow—from concept to clinic.