Anti-INADL Rabbit Polyclonal Antibody for WB, ELISA - Q8NI35

Core Product Specifications and Parameters

Biological Background: InaD-like Protein Function and Localization

• The PATJ gene encodes InaD-like protein (also known as INADL, CIPP, hINADL), a multi-PDZ domain scaffolding protein that facilitates the localization of proteins to the cell membrane. Related references: PMID:11927608 PMID:16678097
• INADL is required for proper tight junction formation and epithelial apico-basal polarity; its L27 domain acts as an apical connector for TJP1/ZO1 condensates. Related references: PMID:11927608 PMID:16678097
• Positively regulates epithelial cell microtubule elongation and migration, likely through the localization of PRKCI/aPKC and PAR3D/PAR3 at leading edges.
• May recruit ARHGEF18 to apical cell–cell boundaries, contributing to junctional integrity. Related references: PMID:22006950
• Localizes predominantly at tight junctions, the apical cell membrane, and the perinuclear cytoplasm.
• Expressed in renal tubules, bladder, testis, ovary, intestine, colon, heart, skeletal muscle, pancreas, and cerebellum.
• Classified as a phosphoprotein with alternative splicing; contains multiple PDZ repeats and is part of the reference proteome.
• Predicted molecular weight approximately 196 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 450–680 of human INADL (NP_795352.2); consider verifying the immunizing sequence for isoform specificity in your experimental system.
• This polyclonal antibody is designated for Western blotting (WB) and ELISA; other applications may require individual optimization.
• The antibody cross-reacts with human and mouse INADL; validate performance in the relevant species and tissue before critical assays.
• In WB, the expected band is around 196 kDa; inclusion of a prestained high-molecular-weight marker is recommended.
• For ELISA, consider pairing with a capture/detection antibody after empirical validation of the assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and laboratories with high-quality IVD raw materials, including this polyclonal antibody targeting the tight junction scaffold INADL. As a comprehensive B2B partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from research to regulated use. Our portfolio supports seamless procurement for projects focused on cell polarity, barrier function, and related disease areas.