Anti-Importin Alpha 5/KPNA1 Rabbit mAb - P52294

Core Product Specifications and Parameters

Biological Background: Importin Alpha 5 Function and Localization

• Importin alpha-5 (KPNA1) is a member of the importin alpha family of nuclear transport receptors and is also referred to as karyopherin subunit alpha-1, nucleoprotein interactor 1 (NPI-1), RAG cohort protein 2, and SRP1-beta.
• It serves as an adapter that recognizes classical nuclear localization signals (NLS) on cargo proteins and docks them to importin beta (KPNB1) for transport through the nuclear pore complex, a process essential for many cellular functions. Related references: PMID:27713473, PMID:7892216, PMID:8692858
• The import complex is translocated via a Ran-GTP-dependent mechanism, with the directionality of transport governed by the asymmetric distribution of Ran-GTP across the nuclear envelope. Related references: PMID:7892216
• KPNA1 also participates in the nuclear import of the PR-DUB complex component BAP1, acting redundantly with KPNA2 and TNPO1. Related references: PMID:35446349
• In the context of infection, KPNA1 can mediate the nuclear import of human cytomegalovirus UL84 protein by recognizing a non-classical NLS.
• Subcellularly, KPNA1 shuttles between the cytoplasm and nucleus, consistent with its transport role.
• It is expressed ubiquitously across human tissues.
• Post-translational modifications including acetylation and phosphorylation have been identified, and the protein is a target for ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human KPNA1, which contains the importin-beta binding (IBB) domain; consider this when selecting detection reagents or designing competition assays.
• The antibody is validated for WB, IF/ICC, IF-P, IHC-P, and ELISA applications on human, mouse, and rat samples; always validate in your specific sample system and optimize antibody dilutions.
• For IF assays, note that KPNA1 shuttles between cytoplasm and nucleus; permeabilization protocols may require optimization to clearly visualize both compartments.
• As a monoclonal antibody, it offers consistent lot-to-lot performance suitable for quantitative comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a streamlined supply chain for IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. For nuclear transport targets like KPNA1, our portfolio supports assay development with optimized antibodies and rare-specificity raw materials, ensuring reliable sourcing from concept to clinic.