Anti-Importin 9 / RANBP9 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96P70

Core Product Specifications and Parameters

Biological Background: Importin 9 / RANBP9 Function and Localization

• Importin-9 (IPO9), also known as Ran-binding protein 9 (RanBP9), is a nuclear transport receptor belonging to the importin beta family.
• It mediates the nuclear import of proteins containing nuclear localization signals (NLS), including histones (H2A, H2B, H4), proteasomes, and actin. Related references: PMID:11823430, PMID:30855230, PMID:34711951
• The import cycle involves docking to nucleoporins, translocation, and Ran-dependent release, ensuring directionality via the cytoplasmic/nuclear Ran GTP/GDP gradient. Related references: PMID:11823430
• Importin-9 acts as a chaperone for histones, preventing inappropriate non-nucleosomal interactions, highlighting a role beyond transport. Related references: PMID:30855230
• It mediates nuclear import of pre-assembled proteasomes through AKIRIN2, which bridges IPO9 and the proteasome complex. Related references: PMID:11823430, PMID:34711951
• Subcellularly, Importin-9 shuttles between the cytoplasm and nucleus.
• Additional post-translational modifications include acetylation (by keyword evidence), though functional details remain to be characterized.

Experimental Guidance and Technical Tips

• The immunogen peptide (residues 50–150) targets the N-terminal region of human Importin-9, which is highly conserved in mouse and rat, supporting the reported cross-reactivity.
• For Western blotting, expect a band at approximately 116 kDa; validate antibody specificity using appropriate positive and negative controls.
• In IHC-P, optimize antigen retrieval and primary antibody concentration for your tissue type; test on tissue known to express Importin-9 (e.g., proliferating cells).
• For ELISA, the antibody can be used as a capture or detection reagent; perform a checkerboard titration to establish optimal working concentrations.
• This monoclonal antibody offers consistent batch-to-batch performance suitable for quantitative analyses.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides integrated access to high-quality antibodies like this Importin-9 (RANBP9) rabbit mAb, along with validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents. Our offering supports diagnostic manufacturers and research laboratories in accelerating assay development for nuclear transport and chromosome biology applications. Contact us to leverage our comprehensive sourcing capabilities from concept to clinic.