Anti-IMPDH2 Polyclonal Antibody for WB, IHC-P, ELISA - P12268

Core Product Specifications and Parameters

Biological Background: IMPDH2 Function and Localization

• IMPDH2 (inosine-5'-monophosphate dehydrogenase 2) is also known as IMPD2 or IMPDH-II, a member of the IMPDH family.
• Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in de novo guanine nucleotide synthesis, crucial for cell growth regulation. Related references: PMID:7763314, PMID:7903306
• Possesses single-stranded nucleic acid-binding activity and may play a role in RNA and/or DNA metabolism. Related references: PMID:14766016
• May contribute to malignancy development and tumor growth progression.
• Localizes to the cytoplasm, nucleus, and cytosol.
• IMPDH2 is the predominant isoform in tumors, while IMPDH1 predominates in normal leukocytes.
• Contains conserved domains including CBS domains and requires NAD and metal ions for its oxidoreductase activity.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitination, potentially regulating its function.

Experimental Guidance and Technical Tips

• For Western blot, consider the predicted molecular weight of ~56 kDa and validate detection using lysates from human, mouse, or rat cell lines.
• In IHC-P, optimal antigen retrieval conditions and antibody dilution should be empirically determined.
• For ELISA, the recombinant immunogen (amino acids 215-514) may serve as a positive control; cross-reactivity with endogenous IMPDH2 should be confirmed.
• Given the high homology among species, this antibody could be suitable for cross-species studies, but validation in the target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw materials. For IMPDH2-related research—spanning nucleotide metabolism and tumor biology—we provide tailored procurement solutions from concept to clinic. Our supply chain expertise supports diagnostic manufacturers, reference laboratories, and research institutes in accelerating assay development and deployment.