Anti-IMPDH2 Polyclonal Antibody for WB, IF/ICC, ELISA - P12268

Core Product Specifications and Parameters

Biological Background: IMPDH2 Function and Localization

• IMPDH2 (inosine-5'-monophosphate dehydrogenase 2) is a key enzyme that catalyzes the conversion of IMP to XMP, the first committed and rate-limiting step in de novo guanine nucleotide biosynthesis, thereby regulating cell growth (PubMed:7763314, PubMed:7903306). Related references: PMID:7763314 PMID:7903306
• The protein also exhibits single-stranded nucleic acid-binding activity and is suggested to participate in RNA and/or DNA metabolism (PubMed:14766016). Related references: PMID:14766016
• IMPDH2 has been implicated in the development of malignancy and the progression of certain tumors. Tissue specificity data show that IMPDH2 predominates over IMPDH1 in tumor cells, contrasting with normal leukocytes where IMPDH1 is the main form.
• Subcellularly, IMPDH2 localizes to the cytoplasm and nucleus, consistent with its roles in nucleotide synthesis and nucleic acid interactions.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and contains CBS domains that may regulate its activity via metal ions such as potassium.
• Keywords associated with IMPDH2 include: CBS domain, DNA-binding, GMP biosynthesis, metal-binding, NAD, oxidoreductase, phosphoprotein, purine biosynthesis, RNA-binding, and Ubl conjugation, highlighting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fragment corresponding to amino acids 215-514 of human IMPDH2; this region may encompass critical catalytic and regulatory domains, potentially contributing to strong antibody reactivity.
• Based on its applications in Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA, the antibody is suitable for detecting IMPDH2 across multiple assay platforms. For WB, the predicted molecular weight is ~56 kDa; always validate with appropriate positive and negative controls in your sample system.
• Given cross-reactivity with human, mouse, and rat, this antibody can be used for comparative studies across species. However, we recommend verifying reactivity in your specific tissue or cell type, considering the known tissue-specific differences between IMPDH1 and IMPDH2 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering access to validated antibodies, bulk ancillary reagents, and rare target sourcing for manufacturers and research institutes. For targets like IMPDH2, critical in nucleotide metabolism and cancer biology, we can connect you with optimized monoclonal and polyclonal antibody supplies, complemented by technical consultation to streamline your assay development from concept to clinic.