Anti-IMPA1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P29218

Core Product Specifications and Parameters

Biological Background: IMPA1 Function and Localization

• IMPA1 (inositol monophosphatase 1) catalyzes the dephosphorylation of myo-inositol monophosphates to generate myo-inositol, a critical precursor for phosphatidylinositols and polyphosphoinositides (PubMed:17068342, PubMed:8718889, PubMed:9462881). Related references: PMID:17068342, PMID:8718889, PMID:9462881
• The enzyme also dephosphorylates other sugar-phosphates in vitro, including D-galactose-1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate and 2′-AMP (PubMed:17068342, PubMed:8718889, PubMed:9462881). Related references: PMID:17068342, PMID:8718889, PMID:9462881
• IMPA1 is a pharmacological target for lithium (Li+) in brain, and its inhibition may underlie lithium's therapeutic effect in bipolar disorder (PubMed:17068342). Related references: PMID:17068342
• It is essential for maintaining inositol homeostasis and normal brain function; mutations are associated with intellectual disability (PubMed:26416544, PubMed:8718889). Related references: PMID:26416544, PMID:8718889
• The protein localizes predominantly to the cytoplasm.
• Post-translational modifications include phosphorylation; it requires magnesium ions for activity and is metal-binding.
• Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen consists of the full-length recombinant human IMPA1 protein (amino acids 1–277), suggesting the antibody can detect both native and denatured forms; consider this when optimizing for different applications.
• For Western blot (WB), an expected band at approximately 30 kDa should be observed; validate in the relevant human, mouse, or rat tissue lysates.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), since IMPA1 localizes to the cytoplasm, permeabilization steps are required; antigen retrieval methods may influence staining patterns.
• For ELISA, the polyclonal nature of the antibody may provide robust detection; consider pairing with a specific capture antibody or direct antigen coating.

CamelBio: Your One-Stop Sourcing Bridge

IMPA1 is a key target in neuroscience and bipolar disorder research. At CamelBio, we offer this IMPA1 polyclonal antibody alongside a comprehensive portfolio of IVD raw materials, including validated antibody pairs and ancillary reagents, to support your diagnostic and research programs from discovery to clinic. Our sourcing expertise ensures timely access to high-quality antibodies for rare targets like IMPA1.