Anti-IMMT Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC - Q16891

Core Product Specifications and Parameters

Biological Background: IMMT/Mitofilin Function and Localization

• Encoded by the IMMT gene, Mitofilin (also designated MIC60, p87/89, or MINOS2) is the core subunit of the mitochondrial contact site and cristae organizing system (MICOS) complex.
• As a critical component of the MICOS complex, IMMT is essential for maintaining crista junction integrity, shaping inner membrane architecture, and mediating contact sites with the outer membrane. Related references: PMID:22114354 PMID:25781180 PMID:32567732
• IMMT plays a key role in stabilizing the MICOS complex and preserving normal mitochondrial cristae morphology. Related references: PMID:22114354 PMID:25781180 PMID:32567732
• The protein localizes exclusively to the mitochondrial inner membrane.
• Post-translational modifications include phosphorylation and acetylation; the protein is subject to alternative splicing and has been implicated in host-virus interactions.

Experimental Guidance and Technical Tips

• For western blot analysis, the expected band is approximately 84 kDa; consider using whole cell or mitochondrial lysates from human, mouse, or rat tissues/cells and validate with appropriate controls.
• When performing immunohistochemistry (IHC-P), optimal results may require antigen retrieval and titration of the primary antibody for each tissue type.
• For immunofluorescence microscopy (IF/ICC), include fixed-cell controls and confirm mitochondrial localization; co-staining with mitochondrial markers is recommended.
• This antibody has been shown to work in ELISA; consider standard indirect ELISA protocols with recombinant or native antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material partner, providing diagnostic developers and researchers with access to high-quality antibodies, such as this anti-IMMT polyclonal antibody for mitochondrial biology and disease studies. Our portfolio includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customizable sourcing solutions to accelerate your assay development from concept to clinic. Contact us to discuss your specific needs and streamline your supply chain.