Anti-IMMP2L Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96T52

Core Product Specifications and Parameters

Biological Background: IMMP2L Function and Localization

• IMMP2L (IMP2-like protein) is a mitochondrial inner membrane protease subunit belonging to the peptidase S26 family.
• It functions as a hydrolase that catalyzes the removal of transit peptides from nuclear-encoded mitochondrial proteins, facilitating their correct localization across the inner membrane into the intermembrane space.
• One known substrate is DIABLO/Smac, a pro-apoptotic protein released from mitochondria during apoptosis, highlighting IMMP2L's role in mitochondrial protein maturation.
• The protein is localized to the mitochondrial inner membrane and contains transmembrane helices consistent with its membrane-bound protease activity.
• Tissue expression is broad, with expression detected in all tested tissues except adult liver and lung, suggesting a ubiquitous mitochondrial housekeeping role.
• Alternative splicing of the IMMP2L gene results in multiple isoforms, which may contribute to observed protein weight variations (predicted ~19.7 kDa; also annotated as 12kDa/19kDa from product data).
• Keywords: Alternative splicing, Hydrolase, Membrane, Mitochondrion inner membrane, Protease, Transmembrane, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the mature IMMP2L protein (aa 38–175), which may recognize multiple isoforms; confirm specificity in the relevant sample system.
• For Western blot, consider detecting bands around 12 kDa and 19 kDa corresponding to known isoforms; validate with positive controls expressing IMMP2L (e.g., HeLa, HEK293).
• In immunocytochemistry, mitochondrial staining can be confirmed by co-localization with mitochondrial markers; optimize fixation/permeabilization conditions.
• For ELISA applications, the antibody may be used for capture or detection; pairing with another clone or antibody may be required for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With this IMMP2L rabbit polyclonal antibody, researchers targeting mitochondrial processing, protein import, or apoptosis pathways can source high-quality reagents supported by technical expertise. Our supply capabilities extend to rare targets and custom development, streamlining your journey from concept to clinic.