Anti-ILT5/CD85a (LILRB3) Monoclonal Antibody () for Flow Cytometry - O75022

Core Product Specifications and Parameters

Biological Background: ILT5/CD85a Function and Localization

• LILRB3 (also known as ILT5, CD85a, LIR3) is a member of the leukocyte immunoglobulin-like receptor subfamily B.
• Functions as a receptor for class I MHC antigens and contains immunoreceptor tyrosine-based inhibitory motifs (ITIM) in its intracellular domain.
• Upon co-ligation with immune receptors such as FCGR2B and the B-cell receptor, LILRB3 down-regulates antigen-induced B-cell activation by recruiting phosphatases.
• Predominantly expressed on the cell membrane of monocytes and B-cells.
• The protein undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation.
• The molecular weight is approximately 69 kDa (canonical isoform), with a minor isoform at 71 kDa.

Experimental Guidance and Technical Tips

• This -conjugated antibody is designed for direct flow cytometry detection of human ILT5/CD85a on live cells.
• Consider using appropriate isotype controls and Fc receptor blocking steps to reduce non-specific binding.
• The immunogen corresponds to the extracellular region (aa 24-418), which contains multiple Ig-like domains; thus, the antibody is likely to recognize the native conformation of the receptor.
• Titrate the antibody to determine the optimal concentration for your specific experimental conditions.
• Validate the staining pattern using known ILT5-expressing cell lines or primary monocytes and B-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this high-quality anti-ILT5/CD85a monoclonal antibody, to support immunology research and diagnostic development. As a one-stop sourcing partner, we provide validated antibody pairs, bulk ancillary reagents, and customized raw-material solutions to help streamline your assay development from concept to clinic.