Anti-ILKAP Polyclonal Antibody for WB and ELISA - Q9H0C8

Core Product Specifications and Parameters

Biological Background: ILKAP Function and Localization

• ILKAP (Integrin-linked kinase-associated serine/threonine phosphatase 2C) belongs to the PP2C family of protein phosphatases and is also known as PP2C-DELTA, PP2CD, PPM1O, ILKAP2, and ILKAP3.
• It selectively associates with integrin-linked kinase (ILK), modulating cell adhesion and growth factor signaling, and inhibits the ILK-GSK3B signaling axis.
• Plays a role in cell cycle progression by dephosphorylating substrates critical for cell proliferation; may inhibit oncogenic transformation.
• Localizes primarily to the cytoplasm.
• Widely expressed across tissues, with highest levels in striated muscle and lower levels in various smooth muscle tissues.
• Requires metal cofactors such as magnesium or manganese for catalytic activity; is subject to acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-392) covers nearly the full-length ILKAP protein, suggesting the antibody may detect endogenous full-length and potential isoforms.
• For Western blotting, use reducing conditions and appropriate loading controls; the predicted molecular weight is 43 kDa.
• Validate the antibody in the relevant sample system, as expression is highest in striated muscle and lower in smooth muscle.
• Cross-reactivity with mouse enables verification in mouse tissue or cell culture models.
• For ELISA applications, optimize coating antigen concentration and detection conditions for your specific setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with reliable access to validated antibody pairs, optimized polyclonal antibodies like this anti-ILKAP product, and bulk ancillary reagents. Whether your work focuses on cell adhesion, growth factor signaling, or cancer-related phosphatase research, our one-stop platform bridges the gap from raw material sourcing to clinical application, supporting every stage of assay development.